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6.1.1.24: glutamate-tRNAGln ligase

This is an abbreviated version!
For detailed information about glutamate-tRNAGln ligase, go to the full flat file.

Word Map on EC 6.1.1.24

Reaction

ATP
+
L-glutamate
 +
tRNAGlx
=
AMP
 +
diphosphate
 +
glutamyl-tRNAGlx

Synonyms

Glu-Q-RS, GluRS, GluRS1, GluRS2, GluRSND, glutamyl-queuosine tRNAAsp synthetase, Glutamyl-tRNA synthetase, GlxRS, ND-GluRS, non-discriminating GluRS, non-discriminating glutamyl-tRNA synthetase, nondiscriminating GluRS, nondiscriminating glutamyl-tRNA synthetase, Pf3D7_1357200, TM1875

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.24 glutamate-tRNAGln ligase

Purification

Purification on EC 6.1.1.24 - glutamate-tRNAGln ligase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native ND-GluRS by gel filtration
-
recombinant C-terminally and N-terminally His6-tagged ND-GluRS from Escherichia coli strain BL21 by nickel affinity and anion exchange chromatography, and gel filtration to homogeneity
Thermosynechococcus vestitus
-
recombinant Glu-Q-RS from Escherichia coli strain BL21(DE3) by anion exchange and hydroxyapatite chromatography, followed by dialysis, presence of 10% glycerol during the purification prevents the precipitation of Glu-Q-RS
-
recombinant GluRSND from Escherichia coli pLysS Rosetta cells
-
recombinant TM1875 from Escherichia coli strain Rosetta2 (DE3) by anion exchange chromatography and gel filtration
using ion-exchange column chromatography and gel-filtration