6.1.1.20: phenylalanine-tRNA ligase

This is an abbreviated version!
For detailed information about phenylalanine-tRNA ligase, go to the full flat file.

Reaction

Synonyms

CML33, ctPheRS, cytoplasmic phenylalanyl-tRNA synthetase, cytosolic phenylalanyl-tRNA synthetase, cytosolic PheRS, EcPheRS, FARS2, FRS, hcPheRS, HSPC173, L-Phenylalanyl-tRNA synthetase, mitochondrial phenylalanyl-tRNA synthetase, mitochondrial PheRS, mitPheRS, More, mtPheRS, p-azido-phenylalanyl-tRNA synthetase, pAzFRS, Phe-RS, Phenylalanine translase, phenylalanine tRNA synthetase, Phenylalanine--tRNA ligase, Phenylalanine-tRNA synthetase, Phenylalanyl transfer ribonucleic acid synthetase, Phenylalanyl-transfer ribonucleate synthetase, Phenylalanyl-transfer RNA ligase, Phenylalanyl-transfer RNA synthetase, Phenylalanyl-tRNA ligase, Phenylalanyl-tRNA synthetase, PheRS, PheRS-alpha, Synthetase, phenylalanyl-transfer ribonucleate

ECTree

     6 Ligases

         6.1 Forming carbon-oxygen bonds

             6.1.1 Ligases forming aminoacyl-tRNA and related compounds

                6.1.1.20 phenylalanine-tRNA ligase

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Results	in table
4	Activating Compound
102634	AA Sequence
3	Application
1	CAS Registry Number
34	Cloned(Commentary)
61	Cofactor
19	Crystallization (Commentary)
186	Disease/ Diagnostics
4	General Information
1	General Stability
112	IC50 Value
292	Inhibitors
32	kcat/KM [mM/s]
135	Ki Value [mM]
157	KM Value [mM]
33	Localization
33	Metals/Ions
74	Molecular Weight [Da]
70	Natural Substrates/ Products (Substrates)
583	Organism
4	Pathway
123	PDB ID
18	pH Optimum
1	pH Range
1	pH Stability
3	pI Value
1	Posttranslational Modification
71	Protein Variants
35	Purification (Commentary)
11	Reaction
65	Reaction Type
155	Reference
36	Source Tissue
20	Specific Activity [micromol/min/mg]
2	Storage Stability
248	Substrates and Products (Substrate)
69	Subunits
490	Synonyms
1	Systematic Name
21	Temperature Optimum [°C]
3	Temperature Range [°C]
10	Temperature Stability [°C]
76	Turnover Number [1/s]

KCat KM Value

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KCat KM Value on EC 6.1.1.20 - phenylalanine-tRNA ligase

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

15500

2-L-naphthylalanine

Saccharomyces cerevisiae

-

mutant enzyme N412G/T415G/S418C/S437F, at pH 7.6, temperature not specified in the publication

743933

130

2-L-tyrosine

Saccharomyces cerevisiae

-

at pH 7.5 and 37°C

745346

0.095 - 0.43

3,4-dihydroxy-L-phenylalanine

3 entries

23

3-L-tyrosine

Saccharomyces cerevisiae

-

at pH 7.5 and 37°C

745346

66 - 70

ATP

2 entries

4.3 - 5

DL-m-tyrosine

2 entries

1.7 - 1400

L-phenylalanine

12 entries

2500

L-tryptophan

Saccharomyces cerevisiae

-

mutant enzyme N412G/T415G/S418C/S437F, at pH 7.6, temperature not specified in the publication

743933

0.017

L-tyrosine

Homo sapiens

O95363

mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C

727288

7.2 - 1000

tRNAPhe

8 entries

0.095

3,4-dihydroxy-L-phenylalanine

Homo sapiens

O95363

mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C

727288

0.37

3,4-dihydroxy-L-phenylalanine

Homo sapiens

O95363

cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C

727288

0.43

3,4-dihydroxy-L-phenylalanine

Thermus thermophilus

-

in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C

727288

66

ATP

Pseudomonas aeruginosa

-

at pH 7.5 and 37°C

744752

70

ATP

Streptococcus pneumoniae

-

at pH 7.5 and 37°C

744752

4.3

DL-m-tyrosine

Homo sapiens

O95363

mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C

727288

5

DL-m-tyrosine

Homo sapiens

O95363

cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C

727288

1.7

L-phenylalanine

Homo sapiens

O95363

mutant enzyme R117G, at pH 8.5 and 37°C

746396

2.2

L-phenylalanine

Homo sapiens

O95363

mutant enzyme H99D, at pH 8.5 and 37°C

746396

8.8

L-phenylalanine

Homo sapiens

O95363

mitochondrial chimeric enzyme with implanted editing module from Escherichia coli phenylalanine-tRNA synthetase, at pH 8.5 and 37°C

746395

28.3

L-phenylalanine

Homo sapiens

O95363

mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C

727288

71.4

L-phenylalanine

Homo sapiens

O95363

wild type mitochondrial enzyme, at pH 8.5 and 37°C

746395

80

L-phenylalanine

Homo sapiens

O95363

wild type enzyme, at pH 8.5 and 37°C

746396

270

L-phenylalanine

Homo sapiens

O95363

cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C

727288

340

L-phenylalanine

Pseudomonas aeruginosa

-

at pH 7.5 and 37°C

744752

650

L-phenylalanine

Thermus thermophilus

-

in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C

727288

863

L-phenylalanine

Saccharomyces cerevisiae

-

mutant enzyme N412G/T415G/S418C/S437F, at pH 7.6, temperature not specified in the publication

743933

1100

L-phenylalanine

Streptococcus pneumoniae

-

at pH 7.5 and 37°C

744752

1400

L-phenylalanine

Saccharomyces cerevisiae

-

at pH 7.5 and 37°C

745346

7.2

tRNAPhe

Homo sapiens

O95363

mutant enzyme R383C, at pH 8.5 and 37°C

746396

8.7

tRNAPhe

Homo sapiens

O95363

mutant enzyme D289Y, at pH 8.5 and 37°C

746396

14.3

tRNAPhe

Homo sapiens

O95363

mutant enzyme R387Q, at pH 8.5 and 37°C

746396

23.3

tRNAPhe

Homo sapiens

O95363

mutant enzyme T210M, at pH 8.5 and 37°C

746396

64

tRNAPhe

Pseudomonas aeruginosa

-

at pH 7.5 and 37°C

744752

125

tRNAPhe

Homo sapiens

O95363

mutant enzyme P49A, at pH 8.5 and 37°C

746396

160

tRNAPhe

Homo sapiens

O95363

wild type enzyme, at pH 8.5 and 37°C

746396

1000

tRNAPhe

Streptococcus pneumoniae

-

at pH 7.5 and 37°C

744752