5.5.1.6: chalcone isomerase

This is an abbreviated version!
For detailed information about chalcone isomerase, go to the full flat file.

Reaction

Synonyms

Chalcone isomerase, chalcone isomerase-like protein, Chalcone-flavanone isomerase, CHI, Chi-1, Chi1, CHI1A, CHI1B1, CHI1B2, CHI2, CHI2B1, CHI2B2, Chi3, CHI3A, CHI3A1, CHI3A2, CHI3B, CHI3B1, CHI3C1, CHI3C2, CHI4, CHI4A, CHI4B, CHIL, CHIL1, CHIL2, EFP, Isomerase, chalcone, MpCHI, SlCHI1, taxifolin-isomerizing enzyme, TRANSPARENT TESTA 5 protein, TT5, type IV CHI protein

ECTree

     5 Isomerases

         5.5 Intramolecular lyases

             5.5.1 Intramolecular lyases (only sub-subclass identified to date)

                5.5.1.6 chalcone isomerase

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Results	in table
1	Activating Compound
264	AA Sequence
5	Application
1	CAS Registry Number
36	Cloned(Commentary)
4	Crystallization (Commentary)
9	Expression
18	General Information
1	General Stability
48	Inhibitors
34	kcat/KM [mM/s]
73	KM Value [mM]
17	Localization
22	Molecular Weight [Da]
30	Natural Substrates/ Products (Substrates)
170	Organism
10	Pathway
54	PDB ID
9	pH Optimum
4	pH Range
3	pI Value
1	Posttranslational Modification
20	Protein Variants
18	Purification (Commentary)
2	Reaction
1	Reaction Type
103	Reference
96	Source Tissue
7	Specific Activity [micromol/min/mg]
3	Storage Stability
114	Substrates and Products (Substrate)
28	Subunits
89	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
65	Turnover Number [1/s]

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

35.5 - 96.8

2',4'-dihydroxy-4-methylchalcone

2 entries

42 - 45300

2',4'-dihydroxychalcone

3 entries

37.4 - 59.9

2'-hydroxychalcone

2 entries

670800 - 671000

4,2',4',6'-tetrahydrochalcone

2 entries

1.87 - 833

4,2',4',6'-Tetrahydroxychalcone

6 entries

135000

4,2',4'-trihydrochalcone

Medicago sativa

P28012

pH 7.5, 25°C

652235

51.3 - 183

4,2',4'-trihydroxychalcone

5 entries

32.5 - 62.5

4,2',5'-trihydroxychalcone

2 entries

5526 - 5530

4,2'-dihydrochalcone

2 entries

22.6 - 57.4

4,2'-dihydroxychalcone

2 entries

0.44 - 7.3

6'-Deoxychalcone

4 entries

70.4 - 108.7

6'-hydroxychalcone

4 entries

48.33

butein

Eubacterium ramulus

V9P0A9

-

660833

0.002 - 38.33

isoliquiritigenin

11 entries

0.07 - 2300

naringenin chalcone

17 entries

additional information

additional information

Glycine max

-

-

3725

-

35.5

2',4'-dihydroxy-4-methylchalcone

Glycine max

Q53B70, Q53B74, Q93XE6

pH 8, 20°C

663135

96.8

2',4'-dihydroxy-4-methylchalcone

Glycine max

Q53B70, Q53B74, Q93XE6

pH 8, 20°C

663135

42

2',4'-dihydroxychalcone

Glycine max

Q53B70, Q53B74, Q93XE6

pH 8, 20°C

663135

86.3

2',4'-dihydroxychalcone

Glycine max

Q53B70, Q53B74, Q93XE6

pH 8, 20°C

663135

45300

2',4'-dihydroxychalcone

Medicago sativa

P28012

pH 7.5, 25°C

652235

37.4

2'-hydroxychalcone

Glycine max

Q53B70, Q53B74, Q93XE6

pH 8, 20°C

663135

59.9

2'-hydroxychalcone

Glycine max

Q53B70, Q53B74, Q93XE6

pH 8, 20°C

663135

670800

4,2',4',6'-tetrahydrochalcone

Medicago sativa

P28012

pH 7.5, 25°C

652235

671000

4,2',4',6'-tetrahydrochalcone

Medicago sativa

P28012

pH 7.5, 25°C

652235

1.87

4,2',4',6'-Tetrahydroxychalcone

Citrus sinensis

-

-

3735

152.2

4,2',4',6'-Tetrahydroxychalcone

Glycine max

Q53B70, Q53B74, Q93XE6

pH 8, 20°C

663135

219.1

4,2',4',6'-Tetrahydroxychalcone

Glycine max

Q53B70, Q53B74, Q93XE6

pH 8, 20°C

663135

478.8

4,2',4',6'-Tetrahydroxychalcone

Glycine max

Q53B70, Q53B74, Q93XE6

pH 8, 20°C

663135

700

4,2',4',6'-Tetrahydroxychalcone

Glycine max

-

methanethiolated enzyme

3739

833

4,2',4',6'-Tetrahydroxychalcone

Glycine max

-

native enzyme

3739

51.3

4,2',4'-trihydroxychalcone

Glycine max

Q53B70, Q53B74, Q93XE6

pH 8, 20°C

663135

84.5

4,2',4'-trihydroxychalcone

Glycine max

Q53B70, Q53B74, Q93XE6

pH 8, 20°C

663135

107

4,2',4'-trihydroxychalcone

Glycine max

-

methanethiolated enzyme

3739

167

4,2',4'-trihydroxychalcone

Glycine max

-

native enzyme

3739

183

4,2',4'-trihydroxychalcone

Glycine max

-

-

3734

32.5

4,2',5'-trihydroxychalcone

Glycine max

Q53B70, Q53B74, Q93XE6

pH 8, 20°C

663135

62.5

4,2',5'-trihydroxychalcone

Glycine max

Q53B70, Q53B74, Q93XE6

pH 8, 20°C

663135

5526

4,2'-dihydrochalcone

Medicago sativa

P28012

pH 7.5, 25°C

652235

5530

4,2'-dihydrochalcone

Medicago sativa

P28012

pH 7.5, 25°C

652235

22.6

4,2'-dihydroxychalcone

Glycine max

Q53B70, Q53B74, Q93XE6

pH 8, 20°C

663135

57.4

4,2'-dihydroxychalcone

Glycine max

Q53B70, Q53B74, Q93XE6

pH 8, 20°C

663135

0.44

6'-Deoxychalcone

Selaginella moellendorffii

-

at pH 7.5 and 35°C

748773

0.76

6'-Deoxychalcone

Physcomitrella appendiculatum

-

at pH 7.5 and 35°C

748773

7.06

6'-Deoxychalcone

Medicago sativa

-

at pH 7.5 and 35°C

748773

7.3

6'-Deoxychalcone

Marchantia paleacea

-

at pH 7.5 and 35°C

748773

70.4

6'-hydroxychalcone

Selaginella moellendorffii

-

at pH 7.5 and 35°C

748773

75.6

6'-hydroxychalcone

Physcomitrella appendiculatum

-

at pH 7.5 and 35°C

748773

103

6'-hydroxychalcone

Medicago sativa

-

at pH 7.5 and 35°C

748773

108.7

6'-hydroxychalcone

Marchantia paleacea

-

at pH 7.5 and 35°C

748773

0.002

isoliquiritigenin

Deschampsia antarctica

G4U3G8

mutant enzyme R34A, at pH 7.6 and 25°C

749122

0.002

isoliquiritigenin

Deschampsia antarctica

G4U3G8

mutant enzyme R34M, at pH 7.6 and 25°C

749122

0.003

isoliquiritigenin

Deschampsia antarctica

G4U3G8

mutant enzyme K108A, at pH 7.6 and 25°C

749122

0.003

isoliquiritigenin

Deschampsia antarctica

G4U3G8

mutant enzyme S189A, at pH 7.6 and 25°C

749122

0.005

isoliquiritigenin

Deschampsia antarctica

G4U3G8

mutant enzyme I190M, at pH 7.6 and 25°C

749122

0.007

isoliquiritigenin

Deschampsia antarctica

G4U3G8

mutant enzyme Y116A, at pH 7.6 and 25°C

749122

0.008

isoliquiritigenin

Deschampsia antarctica

G4U3G8

mutant enzyme M36A, at pH 7.6 and 25°C

749122

0.017

isoliquiritigenin

Deschampsia antarctica

G4U3G8

mutant enzyme S189T, at pH 7.6 and 25°C

749122

0.02

isoliquiritigenin

Deschampsia antarctica

G4U3G8

wild type enzyme, at pH 7.6 and 25°C

749122

0.023

isoliquiritigenin

Deschampsia antarctica

G4U3G8

mutant enzyme Q104E, at pH 7.6 and 25°C

749122

38.33

isoliquiritigenin

Eubacterium ramulus

V9P0A9

-

660833

0.07

naringenin chalcone

Eubacterium ramulus

V9P0A9

mutant enzyme H33Q, at pH 6.4, temperature not specified in the publication

746629

0.2

naringenin chalcone

Eubacterium ramulus

V9P0A9

mutant enzyme H33E, at pH 6.4, temperature not specified in the publication

746629

0.6

naringenin chalcone

Eubacterium ramulus

V9P0A9

mutant enzyme H33A, at pH 6.4, temperature not specified in the publication

746629

5

naringenin chalcone

Deschampsia antarctica

G4U3G8

mutant enzyme S189A, at pH 7.6 and 25°C

749122

7.8

naringenin chalcone

Deschampsia antarctica

G4U3G8

mutant enzyme M96K, at pH 7.6 and 25°C

749122

9.6

naringenin chalcone

Deschampsia antarctica

G4U3G8

mutant enzyme Y116A, at pH 7.6 and 25°C

749122

35.2

naringenin chalcone

Deschampsia antarctica

G4U3G8

mutant enzyme M36A, at pH 7.6 and 25°C

749122

56.9

naringenin chalcone

Deschampsia antarctica

G4U3G8

mutant enzyme K108A, at pH 7.6 and 25°C

749122

130.3

naringenin chalcone

Deschampsia antarctica

G4U3G8

wild type enzyme, at pH 7.6 and 25°C

749122

134.7

naringenin chalcone

Deschampsia antarctica

G4U3G8

mutant enzyme Q104E, at pH 7.6 and 25°C

749122

197.7

naringenin chalcone

Deschampsia antarctica

G4U3G8

mutant enzyme I190M, at pH 7.6 and 25°C

749122

228.2

naringenin chalcone

Deschampsia antarctica

G4U3G8

mutant enzyme S189T, at pH 7.6 and 25°C

749122

784

naringenin chalcone

Eubacterium ramulus

V9P0A9

mutant enzyme Y48F, at pH 6.4, temperature not specified in the publication

746629

1142

naringenin chalcone

Eubacterium ramulus

V9P0A9

wild type enzyme, at pH 6.4, temperature not specified in the publication

746629

1313

naringenin chalcone

Eubacterium ramulus

V9P0A9

mutant enzyme H73A, at pH 6.4, temperature not specified in the publication

746629

1379

naringenin chalcone

Eubacterium ramulus

V9P0A9

mutant enzyme R125A, at pH 6.4, temperature not specified in the publication

746629

2300

naringenin chalcone

Eubacterium ramulus

V9P0A9

-

660833