5.4.99.28: tRNA pseudouridine32 synthase
This is an abbreviated version!
For detailed information about tRNA pseudouridine32 synthase, go to the full flat file.
Word Map on EC 5.4.99.28
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5.4.99.28
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synthases
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rrna
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dual-specificity
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pseudouridylating
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5-fluorouridine
- 5.4.99.28
- synthases
- rrna
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dual-specificity
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pseudouridylating
- 5-fluorouridine
Reaction
Synonyms
pseudouridine synthase RluA, Pus9, Rib2/Pus8p, RluA, tRNA:Psi32-synthase
ECTree
5.4.99.28 tRNA pseudouridine32 synthaseAdvanced search results
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results (Engineering
Engineering on EC 5.4.99.28 - tRNA pseudouridine32 synthase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
C117A
kcat is 1.4fold lower than wild-type value, KM is 3.9fold higher than wild-type value
C117A/A128A
kcat is 1.3fold lower than wild-type value, KM is 1.5fold higher than wild-type value
C128A
kcat is 1.5fold higher than wild-type value, KM is 1.5fold higher than wild-type value
P29G
kcat-value is 1.6fold lower than wild-type value. KM-value is 2.9fold higher than wild-type value
P29L
kcat-value is 1.9fold lower than wild-type value. KM-value is 4.2fold higher than wild-type value
D112A
mutant does not pseudouridine32 in formation in cytoplasmic tRNAGly of the DELTArib2/pus8/DELTApus9 strain
additional information
additional information
substitution of cysteine for amino acids with nonnucleophilic side chains does not significantly alter the catalytic activity
additional information
Pus9P lacking the 23 N-terminal amino acids is inactive
additional information
Pus9P lacking the 23 N-terminal amino acids is inactive
additional information
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Pus9P lacking the 23 N-terminal amino acids is inactive
