5.4.99.28: tRNA pseudouridine32 synthase
This is an abbreviated version!
For detailed information about tRNA pseudouridine32 synthase, go to the full flat file.
Word Map on EC 5.4.99.28
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5.4.99.28
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synthases
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rrna
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dual-specificity
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pseudouridylating
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5-fluorouridine
- 5.4.99.28
- synthases
- rrna
-
dual-specificity
-
pseudouridylating
- 5-fluorouridine
Reaction
Synonyms
pseudouridine synthase RluA, Pus9, Rib2/Pus8p, RluA, tRNA:Psi32-synthase
ECTree
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General Information
General Information on EC 5.4.99.28 - tRNA pseudouridine32 synthase
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malfunction
the rluA deletion mutant fails to form either 23S RNA pseudouridine746 or tRNA pseudouridine32. Replacement of rluA in trans on a rescue plasmid restores both pseudouridines. There is no difference in exponential growth rate between wild-type and MG1655(rluA-) either in rich or minimal medium at 24, 37, or 42°C, but when both strains are grown together, a strong selection against the deletion strain is observed
malfunction
Rib2/Pus8p acts on cytoplasmic tRNAs, whereas Pus9p modifies the same position in mitochondrial tRNAs. The DELTArib2/pus8/DELTApus9, is viable only in the presence of 15 mg/liter riboflavin in the growth medium