5.4.99.28: tRNA pseudouridine32 synthase
This is an abbreviated version!
For detailed information about tRNA pseudouridine32 synthase, go to the full flat file.
Word Map on EC 5.4.99.28
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5.4.99.28
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synthases
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rrna
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dual-specificity
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pseudouridylating
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5-fluorouridine
- 5.4.99.28
- synthases
- rrna
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dual-specificity
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pseudouridylating
- 5-fluorouridine
Reaction
Synonyms
pseudouridine synthase RluA, Pus9, Rib2/Pus8p, RluA, tRNA:Psi32-synthase
ECTree
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Turnover Number
Turnover Number on EC 5.4.99.28 - tRNA pseudouridine32 synthase
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0.051
pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29L
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0.061
tRNA uridine32
pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29G
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0.068
tRNA uridine32
pH 7.5, 37°C, anticodon stem-loop of Escherichia coli tRNAPhe (17-mer)
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0.07
tRNA uridine32
pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C117A
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0.074
tRNA uridine32
pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C117A/C128A
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0.099
tRNA uridine32
pH 7.5, 37°C, Escherichia coli tRNAPhe, wild-type enzyme
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0.099
tRNA uridine32
pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe. wild-type enzyme
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0.15
tRNA uridine32
pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C128A
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