5.3.1.14: L-rhamnose isomerase
This is an abbreviated version!
For detailed information about L-rhamnose isomerase, go to the full flat file.
Word Map on EC 5.3.1.14
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5.3.1.14
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l-rhamnulose
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d-allose
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isomerization
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d-psicose
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3-epimerase
-
isomerases
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stutzeri
-
rhaas
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l-lyxose
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synthesis
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isomerized
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aldoses
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l-xylulose
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ketoses
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l-mannose
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l-tagatose
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caldicellulosiruptor
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l-ribose
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l-galactose
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epimerized
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industry
-
food industry
- 5.3.1.14
- l-rhamnulose
- d-allose
-
isomerization
- d-psicose
-
3-epimerase
- isomerases
- stutzeri
-
rhaas
- l-lyxose
- synthesis
-
isomerized
- aldoses
- l-xylulose
- ketoses
- l-mannose
- l-tagatose
-
caldicellulosiruptor
- l-ribose
- l-galactose
-
epimerized
- industry
- food industry
Reaction
Synonyms
COB47_0655, Isomerase, L-rhamnose, L-rhamnose isomerase, L-rhamnose ketol-isomerase, L-RhI, L-RI, RhaA, RhaI, Rhamnose isomerase
ECTree
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Purification
Purification on EC 5.3.1.14 - L-rhamnose isomerase
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heat treatment, nucleic acid precipitation, and Q-Sepharose column chromatography
heat treatment, nucleic acid precipitation, and Q-Sepharose ion-exchange chromatography
native enzyme by anion exchange, hydrophobic interaction, and again anion exchange chromatography to apparent homogeneity, recombinant His6-tagged enzyme 3.87fold from Escherichia coli strain JM109 by nickel affinity chromatography to homogeneity
Ni-NTA column chromatography
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and dialysis