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5.3.1.14: L-rhamnose isomerase

This is an abbreviated version!
For detailed information about L-rhamnose isomerase, go to the full flat file.

Word Map on EC 5.3.1.14

Reaction

L-rhamnopyranose
=
L-rhamnulose

Synonyms

COB47_0655, Isomerase, L-rhamnose, L-rhamnose isomerase, L-rhamnose ketol-isomerase, L-RhI, L-RI, RhaA, RhaI, Rhamnose isomerase

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.1 Interconverting aldoses and ketoses, and related compounds
                5.3.1.14 L-rhamnose isomerase

Purification

Purification on EC 5.3.1.14 - L-rhamnose isomerase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
heat treatment, nucleic acid precipitation, and Q-Sepharose column chromatography
heat treatment, nucleic acid precipitation, and Q-Sepharose ion-exchange chromatography
His-Trap column chromatography
-
His-Trap HP column chromatography, and gel filtration
native enzyme by anion exchange, hydrophobic interaction, and again anion exchange chromatography to apparent homogeneity, recombinant His6-tagged enzyme 3.87fold from Escherichia coli strain JM109 by nickel affinity chromatography to homogeneity
Ni-NTA column chromatography
recombinant His-tagged enzyme
-
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and dialysis