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5.3.1.14: L-rhamnose isomerase

This is an abbreviated version!
For detailed information about L-rhamnose isomerase, go to the full flat file.

Word Map on EC 5.3.1.14

Reaction

L-rhamnopyranose
=
L-rhamnulose

Synonyms

COB47_0655, Isomerase, L-rhamnose, L-rhamnose isomerase, L-rhamnose ketol-isomerase, L-RhI, L-RI, RhaA, RhaI, Rhamnose isomerase

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.1 Interconverting aldoses and ketoses, and related compounds
                5.3.1.14 L-rhamnose isomerase

Temperature Stability

Temperature Stability on EC 5.3.1.14 - L-rhamnose isomerase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
50 h stable, partially purified enzyme
40 - 70
the activity is fully retained after 2 h incubation at 40-70°C
60 - 80
Halalkalibacterium halodurans
-
the enzyme shows 100% activity for 10 h at 60°C, the half-life of the enzyme is more than 900 min, about 25 min, and 5 min at 60°C, 70°C, and 80°C, respectively
75 - 95
-
the half-lives of the enzyme at 75°C, 80°C, 85°C, 90°C and 95°C are 773 h, 347 h, 187 h, 118 h, and 65 h, respectively
80 - 90
the enzyme shows good thermostability and retains more than 90% activity after a 6 h incubation at 80°C and pH 7.0-8.0. The enzyme is stable at 85°C for 4 h. However, the enzyme activity drops sharply to 30% after 2 h at 90°C, and the half-life at 90°C is around 65 min
85
half-life 3.3 h, presence of Co2+