5.3.1.14: L-rhamnose isomerase
This is an abbreviated version!
For detailed information about L-rhamnose isomerase, go to the full flat file.
Word Map on EC 5.3.1.14
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5.3.1.14
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l-rhamnulose
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d-allose
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isomerization
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d-psicose
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3-epimerase
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isomerases
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stutzeri
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rhaas
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l-lyxose
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synthesis
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isomerized
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aldoses
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l-xylulose
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ketoses
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l-mannose
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l-tagatose
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caldicellulosiruptor
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l-ribose
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l-galactose
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epimerized
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industry
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food industry
- 5.3.1.14
- l-rhamnulose
- d-allose
-
isomerization
- d-psicose
-
3-epimerase
- isomerases
- stutzeri
-
rhaas
- l-lyxose
- synthesis
-
isomerized
- aldoses
- l-xylulose
- ketoses
- l-mannose
- l-tagatose
-
caldicellulosiruptor
- l-ribose
- l-galactose
-
epimerized
- industry
- food industry
Reaction
Synonyms
COB47_0655, Isomerase, L-rhamnose, L-rhamnose isomerase, L-rhamnose ketol-isomerase, L-RhI, L-RI, RhaA, RhaI, Rhamnose isomerase
ECTree
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Temperature Stability
Temperature Stability on EC 5.3.1.14 - L-rhamnose isomerase
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40 - 70
the activity is fully retained after 2 h incubation at 40-70°C
50
60
60 - 80
Halalkalibacterium halodurans
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the enzyme shows 100% activity for 10 h at 60°C, the half-life of the enzyme is more than 900 min, about 25 min, and 5 min at 60°C, 70°C, and 80°C, respectively
65
70
75
75 - 95
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the half-lives of the enzyme at 75°C, 80°C, 85°C, 90°C and 95°C are 773 h, 347 h, 187 h, 118 h, and 65 h, respectively
80
80 - 90
the enzyme shows good thermostability and retains more than 90% activity after a 6 h incubation at 80°C and pH 7.0-8.0. The enzyme is stable at 85°C for 4 h. However, the enzyme activity drops sharply to 30% after 2 h at 90°C, and the half-life at 90°C is around 65 min
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after incubation at 50°C in glycine-NaOH buffer (pH 9.0) for 1 h, approximately 70% of enzyme activity is retained
50
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after incubation at 50°C in glycineNaOH buffer (pH 9.0) for 1 h, approximately 70% of enzyme activity is retained
60 min, purified recombinant enzyme, pH 7.0, 90% remaining activity
60 min, purified recombinant enzyme, pH 7.0, 50% remaining activity
the half-lives of the free and immobilized enzymes are 28 and 112 h at pH 8.0 and 75°C in the presence of 1 mM Mn2+