Literature summary for 5.3.1.14 extracted from

Yoshida, H.; Yoshihara, A.; Teraoka, M.; Yamashita, S.; Izumori, K.; Kamitori, S.
Structure of L-rhamnose isomerase in complex with L-rhamnopyranose demonstrates the sugar-ring opening mechanism and the role of a substrate sub-binding site (2013), FEBS Open Bio, 3, 35-40.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant enzyme H101N in complex with L-rhamnose and D-allose, vapor diffusion method, using 7-8% (w/v) polyethylene glycol 20000 and 50 mM MES buffer (pH 6.3)	Pseudomonas stutzeri

Protein Variants

Protein Variants	Comment	Organism
D327N	inactive	Pseudomonas stutzeri
H101N	the enzymatic activity of the mutant is decreased	Pseudomonas stutzeri

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.92	-	L-rhamnose	wild type enzyme, pH and temperature not specified in the publication	Pseudomonas stutzeri
2.7	-	D-allose	wild type enzyme, pH and temperature not specified in the publication	Pseudomonas stutzeri
9.4	-	L-rhamnose	mutant enzyme H101N, pH and temperature not specified in the publication	Pseudomonas stutzeri
60	-	D-allose	mutant enzyme H101N, pH and temperature not specified in the publication	Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-Rhamnose	Pseudomonas stutzeri	-	L-Rhamnulose	-	r

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas stutzeri	Q75WH8	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas stutzeri

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-allose	-	Pseudomonas stutzeri	D-psicose	-	r
L-Rhamnose	-	Pseudomonas stutzeri	L-Rhamnulose	-	r

Synonyms

Synonyms	Comment	Organism
L-RhI	-	Pseudomonas stutzeri
Rhamnose isomerase	-	Pseudomonas stutzeri

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
16.8	-	D-allose	wild type enzyme, pH and temperature not specified in the publication	Pseudomonas stutzeri
24.5	-	D-allose	mutant enzyme H101N, pH and temperature not specified in the publication	Pseudomonas stutzeri
192	-	L-rhamnose	mutant enzyme H101N, pH and temperature not specified in the publication	Pseudomonas stutzeri
374	-	L-rhamnose	wild type enzyme, pH and temperature not specified in the publication	Pseudomonas stutzeri

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.41	-	D-allose	mutant enzyme H101N, pH and temperature not specified in the publication	Pseudomonas stutzeri
6.2	-	D-allose	wild type enzyme, pH and temperature not specified in the publication	Pseudomonas stutzeri
20	-	L-rhamnose	mutant enzyme H101N, pH and temperature not specified in the publication	Pseudomonas stutzeri
410	-	L-rhamnose	wild type enzyme, pH and temperature not specified in the publication	Pseudomonas stutzeri

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Release 2023.1 (January 2023)