5.1.3.B12: Agrobacterium tumefaciens D-psicose 3-epimerase

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For detailed information about Agrobacterium tumefaciens D-psicose 3-epimerase, go to the full flat file.

agtu, Atu4750, D-psicose-3-epimerase, DPE, DPEase

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Results	in table
1	AA Sequence
8	Application
7	Cloned(Commentary)
1	Crystallization (Commentary)
2	General Information
9	Inhibitors
38	kcat/KM [mM/s]
38	KM Value [mM]
2	Localization
11	Metals/Ions
5	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
61	Organism
10	pH Optimum
1	pH Range
1	pH Stability
60	Protein Variants
7	Purification (Commentary)
1	Reaction
22	Reference
1	Specific Activity [micromol/min/mg]
34	Substrates and Products (Substrate)
14	Subunits
19	Synonyms
11	Temperature Optimum [°C]
1	Temperature Range [°C]
13	Temperature Stability [°C]
38	Turnover Number [1/s]

Purification on EC 5.1.3.B12 - Agrobacterium tumefaciens D-psicose 3-epimerase

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recombinant engineered Smt3-D-psicose 3-epimerase conjugate from Escherichia coli strain BL21 by nickel affinity and anion exchange chromatography

recombinant enzyme from Kluyveromyces marxianus strain CICC1911 by ammonium sulfate fractionation and nickel affinity chromatography

recombinant His-tagged enzyme from Agrobacterium tumefaciens by nickel affinity chromatography, and dialysis

recombinant His6-tagged enzyme 1.59fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration