Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. It has no significant sequence similarities to D-tagatose 3-epimerase. It is genetically different from other sugar epimerases | Agrobacterium tumefaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 98% inhibition | Agrobacterium tumefaciens | |
EDTA | activity of D-psicose 3-epimerase is 20% after the removal of metal ions by treatment with EDTA | Agrobacterium tumefaciens | |
Zn2+ | 1 mM, 96% inhibition | Agrobacterium tumefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | 2 | D-psicose | pH 8.0, 50°C | Agrobacterium tumefaciens | |
2 | 4 | D-fructose | pH 8.0, 50°C | Agrobacterium tumefaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 2.7fold increase in the epimerization rate from D-fructose to D-psicose | Agrobacterium tumefaciens | |
Mn2+ | 1 mM, 2.7fold increase in the epimerization rate from D-fructose to D-psicose | Agrobacterium tumefaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33071 | - |
4 * 33071, MALDI-TOF MS | Agrobacterium tumefaciens |
33093 | - |
4 * 33093, calculated from sequence | Agrobacterium tumefaciens |
132000 | - |
gel filtration | Agrobacterium tumefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | A9CH28 | - |
- |
Agrobacterium tumefaciens ATCC 33970 | A9CH28 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Agrobacterium tumefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose | the catalytic efficiency of the enzyme for D-psicose is 586fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 32:68 at 30°C. D-Psicose is produced at 230 g/l from 700-g/l D-fructose at 50°C after 100 min, corresponding to a conversion yield of 32.9% | Agrobacterium tumefaciens | D-psicose | - |
r | |
D-fructose | the catalytic efficiency of the enzyme for D-psicose is 586fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 32:68 at 30°C. D-Psicose is produced at 230 g/l from 700-g/l D-fructose at 50°C after 100 min, corresponding to a conversion yield of 32.9% | Agrobacterium tumefaciens ATCC 33970 | D-psicose | - |
r | |
D-psicose | the catalytic efficiency of the enzyme for D-psicose is 586fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 32:68 at 30°C. D-Psicose is produced at 230 g/l from 700-g/l D-fructose at 50°C after 100 min, corresponding to a conversion yield of 32.9% | Agrobacterium tumefaciens | D-fructose | - |
r | |
D-psicose | the catalytic efficiency of the enzyme for D-psicose is 586fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 32:68 at 30°C. D-Psicose is produced at 230 g/l from 700-g/l D-fructose at 50°C after 100 min, corresponding to a conversion yield of 32.9% | Agrobacterium tumefaciens ATCC 33970 | D-fructose | - |
r | |
additional information | no activity on D-fructose-6-phosphate or D-ribulose-5-phosphate | Agrobacterium tumefaciens | ? | - |
? | |
additional information | no activity on D-fructose-6-phosphate or D-ribulose-5-phosphate | Agrobacterium tumefaciens ATCC 33970 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33071, MALDI-TOF MS | Agrobacterium tumefaciens |
tetramer | 4 * 33093, calculated from sequence | Agrobacterium tumefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Agrobacterium tumefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
250 min, stable. Half-life is 1265 min | Agrobacterium tumefaciens |
50 | - |
half-life: 63.5 min | Agrobacterium tumefaciens |
55 | - |
half-life: 8.9 min | Agrobacterium tumefaciens |
60 | - |
half-life: 3.99 min | Agrobacterium tumefaciens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2068 | - |
D-fructose | pH 8.0, 50°C | Agrobacterium tumefaciens | |
2381 | - |
D-psicose | pH 8.0, 50°C | Agrobacterium tumefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Agrobacterium tumefaciens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
85 | - |
D-fructose | pH 8.0, 50°C | Agrobacterium tumefaciens | |
205 | - |
D-psicose | pH 8.0, 50°C | Agrobacterium tumefaciens |