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Literature summary for 5.1.3.B12 extracted from

  • Kim, H.J.; Hyun, E.K.; Kim, Y.S.; Lee, Y.J.; Oh, D.K.
    Characterization of an Agrobacterium tumefaciens D-psicose 3-epimerase that converts D-fructose to D-psicose (2006), Appl. Environ. Microbiol., 72, 981-985.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. It has no significant sequence similarities to D-tagatose 3-epimerase. It is genetically different from other sugar epimerases Agrobacterium tumefaciens

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 98% inhibition Agrobacterium tumefaciens
EDTA activity of D-psicose 3-epimerase is 20% after the removal of metal ions by treatment with EDTA Agrobacterium tumefaciens
Zn2+ 1 mM, 96% inhibition Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1 2 D-psicose pH 8.0, 50°C Agrobacterium tumefaciens
2 4 D-fructose pH 8.0, 50°C Agrobacterium tumefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, 2.7fold increase in the epimerization rate from D-fructose to D-psicose Agrobacterium tumefaciens
Mn2+ 1 mM, 2.7fold increase in the epimerization rate from D-fructose to D-psicose Agrobacterium tumefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33071
-
4 * 33071, MALDI-TOF MS Agrobacterium tumefaciens
33093
-
4 * 33093, calculated from sequence Agrobacterium tumefaciens
132000
-
gel filtration Agrobacterium tumefaciens

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens A9CH28
-
-
Agrobacterium tumefaciens ATCC 33970 A9CH28
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose the catalytic efficiency of the enzyme for D-psicose is 586fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 32:68 at 30°C. D-Psicose is produced at 230 g/l from 700-g/l D-fructose at 50°C after 100 min, corresponding to a conversion yield of 32.9% Agrobacterium tumefaciens D-psicose
-
r
D-fructose the catalytic efficiency of the enzyme for D-psicose is 586fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 32:68 at 30°C. D-Psicose is produced at 230 g/l from 700-g/l D-fructose at 50°C after 100 min, corresponding to a conversion yield of 32.9% Agrobacterium tumefaciens ATCC 33970 D-psicose
-
r
D-psicose the catalytic efficiency of the enzyme for D-psicose is 586fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 32:68 at 30°C. D-Psicose is produced at 230 g/l from 700-g/l D-fructose at 50°C after 100 min, corresponding to a conversion yield of 32.9% Agrobacterium tumefaciens D-fructose
-
r
D-psicose the catalytic efficiency of the enzyme for D-psicose is 586fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 32:68 at 30°C. D-Psicose is produced at 230 g/l from 700-g/l D-fructose at 50°C after 100 min, corresponding to a conversion yield of 32.9% Agrobacterium tumefaciens ATCC 33970 D-fructose
-
r
additional information no activity on D-fructose-6-phosphate or D-ribulose-5-phosphate Agrobacterium tumefaciens ?
-
?
additional information no activity on D-fructose-6-phosphate or D-ribulose-5-phosphate Agrobacterium tumefaciens ATCC 33970 ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 33071, MALDI-TOF MS Agrobacterium tumefaciens
tetramer 4 * 33093, calculated from sequence Agrobacterium tumefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Agrobacterium tumefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
250 min, stable. Half-life is 1265 min Agrobacterium tumefaciens
50
-
half-life: 63.5 min Agrobacterium tumefaciens
55
-
half-life: 8.9 min Agrobacterium tumefaciens
60
-
half-life: 3.99 min Agrobacterium tumefaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2068
-
D-fructose pH 8.0, 50°C Agrobacterium tumefaciens
2381
-
D-psicose pH 8.0, 50°C Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Agrobacterium tumefaciens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
85
-
D-fructose pH 8.0, 50°C Agrobacterium tumefaciens
205
-
D-psicose pH 8.0, 50°C Agrobacterium tumefaciens