5.1.3.B12: Agrobacterium tumefaciens D-psicose 3-epimerase

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For detailed information about Agrobacterium tumefaciens D-psicose 3-epimerase, go to the full flat file.

Reaction

Synonyms

agtu, Atu4750, D-psicose-3-epimerase, DPE, DPEase

ECTree

     5 Isomerases

         5.1 Racemases and epimerases

             5.1.3 Acting on carbohydrates and derivatives

                5.1.3.B12 Agrobacterium tumefaciens D-psicose 3-epimerase

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Results	in table
1	AA Sequence
8	Application
7	Cloned(Commentary)
1	Crystallization (Commentary)
2	General Information
9	Inhibitors
38	kcat/KM [mM/s]
38	KM Value [mM]
2	Localization
11	Metals/Ions
5	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
61	Organism
10	pH Optimum
1	pH Range
1	pH Stability
60	Protein Variants
7	Purification (Commentary)
1	Reaction
22	Reference
1	Specific Activity [micromol/min/mg]
34	Substrates and Products (Substrate)
14	Subunits
19	Synonyms
11	Temperature Optimum [°C]
1	Temperature Range [°C]
13	Temperature Stability [°C]
38	Turnover Number [1/s]

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Cloned on EC 5.1.3.B12 - Agrobacterium tumefaciens D-psicose 3-epimerase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

DNA and amino acid sequence determination and analysis of the D-psicose 3-epimerase gene, recombinant expression in Escherichia coli strain BL21(DE3), co-expression with xylose isomerase gene from Escherichia coli strain MG1665 from plasmid pET-28a-DPE-xylA

Agrobacterium tumefaciens

A9CH28

747663

expression in Escherichia coli. It has no significant sequence similarities to D-tagatose 3-epimerase. It is genetically different from other sugar epimerases

Agrobacterium tumefaciens

A9CH28

726709

gene agtu, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)

Agrobacterium tumefaciens

A9CH28

747664

gene agtu, subcloning in Escherichia coli strain ER2566, expression of recombinant His-tagged enzyme mutant I33L-S213C in Agrobacterium tumefaciens at high level, optimal at optimal at 60x02C, pH 8.5, 4 g/l cells, and 700 g/l D-fructose. The recombinant cells produced 230 g/l D-psicose after 40 min, with a conversion yield of 33% w/w, a volumetric productivity of 345 g/l/h, and a specific productivity of 86.2 g/g/h

Agrobacterium tumefaciens

A9CH28

748254

gene dpe, DNA and amino acid sequence determination and analysis, recombinant expression of the engineered chimeric Smt3-D-psicose 3-epimerase conjugate in Escherichia coli strain BL21

Agrobacterium tumefaciens

A9CH28

747298

gene dpe, functional recombinant expression of the enzyme in thermotolerant Kluyveromyces marxianus strain CICC1911, pRS42H-dpe is transformed into the thermotolerant cells of strain CICC1911 by using the LiAc/salmon sperm carrier DNA/PEG method

Agrobacterium tumefaciens

A9CH28

749427

the gene AAL45544.1 from Agrobacterium tumefaciens str. C58 is modified by artificial synthesis for overexpression in Escherichial coli, expression in Escherichia coli

Agrobacterium tumefaciens

-

727177