5.1.3.B12: Agrobacterium tumefaciens D-psicose 3-epimerase
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For detailed information about Agrobacterium tumefaciens D-psicose 3-epimerase, go to the full flat file.
Reaction
Synonyms
agtu, Atu4750, D-psicose-3-epimerase, DPE, DPEase
ECTree
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Temperature Stability
Temperature Stability on EC 5.1.3.B12 - Agrobacterium tumefaciens D-psicose 3-epimerase
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50
50 - 55
half-lives of whole recombinant cells at 50°C and 55°C are 125 and 177% higher than those of its double-site variant enzyme, respectively. The thermostability of whole cells is higher than that of enzyme
55
60
50
the wild-type, S213C, I33L, and I33L S213C variant enzymes have half-lives of 62, 202, 445, and 1,853 min at 50°C, respectively
50
purified recombinant chimeric enzyme, retains 45% of the initial activity after 12 h
half-life of wild-type enzyme: 10 min, half-lives of mutant enzymes: G67C (132 min), I33L (64 min), S213C (28 min), V96A (18 min), S8T (15 min), S213P (6 min), S213T (5 min), S213M (3.2 min), S213E (2.8 min)
60
graphene oxide-immobilized enzyme DPEase has a half-life of 720 min, while unbound (agtu) DPEase is stable for 3.99 min