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5.1.3.6: UDP-glucuronate 4-epimerase

This is an abbreviated version!
For detailed information about UDP-glucuronate 4-epimerase, go to the full flat file.

Word Map on EC 5.1.3.6

Reaction

UDP-glucuronate
=
UDP-D-galacturonate

Synonyms

AtUGlcAE1, AtUGlcAE2, AtUGlcAE3, Epimerase, uridine diphosphoglucuronate, GAE, GAE1, GAE2, GAE3, GAE4, GAE5, GAE6, OcUGlcAE3, OsUGlcAE3, UDP glucuronic epimerase, UDP-D-galacturonic acid 4-epimerase, UDP-D-glucuronate-4-epimerase, UDP-D-glucuronic acid 4-epimerase, UDP-galacturonate 4-epimerase, UDP-GlcA 4-epimerase, UDP-GlcA 4-epimerase1, UDP-GlcA 4-epimerase2, UDP-GlcA 4-epimerase3, UDP-glucuronic acid 4-epimerase, UDPGLE, UGlcAE, UGlcAE3, uridine diphosphate galacturonate 4-epimerase, Uridine diphospho-D-galacturonic acid 4-epimerase, Uridine diphosphoglucuronate epimerase, Uridine diphosphoglucuronic epimerase, ZmUGlcAE

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.6 UDP-glucuronate 4-epimerase

Cloned

Cloned on EC 5.1.3.6 - UDP-glucuronate 4-epimerase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Agrobacterium tumefaciens
expression in Escherichia coli
expression in Pichia pastoris
gene UGlcAE3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time quantitative PCR enzyme expression analysis, recombinant expression in Pichia pastoris strain GS115 in the microsomal fraction. The recombinant OcUGlcAE3 can form a microsomal protein by the N-terminal membrane anchor sequence, or can be secrete into the extracellular space through the guidance of the signal peptide. OcUGlcAE3 can be expressed in Escherichia coli strain Transetta (DE3) only as truncated enzyme with deletion of a putative transmembrane domain of 128 aa in the N-terminus
isoform GAE3 is expressed in Escherichia coli BL21(DE3) cells
-
isoform GAE6 is expressed in Escherichia coli BL21(DE3) cells
-
no recovery of active recombinant enzyme when full-length AtUGlcAE1 is expressed in Escherichia coli due to the hydrophobic domain that results in un- or misfolded protein in inclusion bodies within Escherichia coli. Recombinant AtUGlcAE1DELTA1-64, lacking the putative transmembrane domain, can be expressed in Escherichia coli
overexpression in Escherichia coli
-
recombinant ZmUGlcAE, lacking the putative transmembrane domain at amino acid position 1-47, is expressed in Escherichia coli