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Literature summary for 5.1.3.6 extracted from

  • Gu, X.; Bar-Peled, M.
    The biosynthesis of UDP-galacturonic acid in plants. Functional cloning and characterization of Arabidopsis UDP-D-glucuronic acid 4-epimerase (2004), Plant Physiol., 136, 4256-4264.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
no recovery of active recombinant enzyme when full-length AtUGlcAE1 is expressed in Escherichia coli due to the hydrophobic domain that results in un- or misfolded protein in inclusion bodies within Escherichia coli. Recombinant AtUGlcAE1DELTA1-64, lacking the putative transmembrane domain, can be expressed in Escherichia coli Arabidopsis thaliana

General Stability

General Stability Organism
KCl or NaCl, above 300 mM, completly inactivates the enzyme, when stored at -20°C for 2 weeks Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
ADP-Ara 2 mM, about 55% inhibition Arabidopsis thaliana
KCl above 300 mM, inhibits activity of recombinant AtUGlcAE1DELTA1-64 Arabidopsis thaliana
NaCl above 300 mM, inhibits activity of recombinant AtUGlcAE1DELTA1-64 Arabidopsis thaliana
UDP 2 mM, about 60% inhibition Arabidopsis thaliana
UDP-Xyl 2 mM, about 60% inhibition Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.72
-
UDP-D-glucuronate
-
Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43500
-
2 * 43500 Arabidopsis thaliana
88000
-
recombinant AtUGlcAE1DELTA1-64, gel filtration Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O22141
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, AtUGlcAE1DELTA1-64 expressed in Escherichia coli Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-D-glucuronate recombinant AtUGlcAE1DELTA1-64 favors the formation of UDP-D-galacturonate over UDP-glucuronate Arabidopsis thaliana UDP-D-galacturonate
-
r

Subunits

Subunits Comment Organism
dimer 2 * 43500 Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AtUGlcAE1
-
Arabidopsis thaliana
UDP-GlcA 4-epimerase
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 42 recombinant AtUGlcAE1DELTA1-64 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
24
-
UDP-glucuronate
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 7.6 recombinant AtUGlcAE1DELTA1-64 Arabidopsis thaliana