Cloned (Comment) | Organism |
---|---|
no recovery of active recombinant enzyme when full-length AtUGlcAE1 is expressed in Escherichia coli due to the hydrophobic domain that results in un- or misfolded protein in inclusion bodies within Escherichia coli. Recombinant AtUGlcAE1DELTA1-64, lacking the putative transmembrane domain, can be expressed in Escherichia coli | Arabidopsis thaliana |
General Stability | Organism |
---|---|
KCl or NaCl, above 300 mM, completly inactivates the enzyme, when stored at -20°C for 2 weeks | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP-Ara | 2 mM, about 55% inhibition | Arabidopsis thaliana | |
KCl | above 300 mM, inhibits activity of recombinant AtUGlcAE1DELTA1-64 | Arabidopsis thaliana | |
NaCl | above 300 mM, inhibits activity of recombinant AtUGlcAE1DELTA1-64 | Arabidopsis thaliana | |
UDP | 2 mM, about 60% inhibition | Arabidopsis thaliana | |
UDP-Xyl | 2 mM, about 60% inhibition | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.72 | - |
UDP-D-glucuronate | - |
Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43500 | - |
2 * 43500 | Arabidopsis thaliana |
88000 | - |
recombinant AtUGlcAE1DELTA1-64, gel filtration | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O22141 | - |
- |
Purification (Comment) | Organism |
---|---|
partial, AtUGlcAE1DELTA1-64 expressed in Escherichia coli | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
stem | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-glucuronate | recombinant AtUGlcAE1DELTA1-64 favors the formation of UDP-D-galacturonate over UDP-glucuronate | Arabidopsis thaliana | UDP-D-galacturonate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43500 | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AtUGlcAE1 | - |
Arabidopsis thaliana |
UDP-GlcA 4-epimerase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 42 | recombinant AtUGlcAE1DELTA1-64 | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24 | - |
UDP-glucuronate | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 7.6 | recombinant AtUGlcAE1DELTA1-64 | Arabidopsis thaliana |