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Literature summary for 5.1.3.6 extracted from

  • Munoz, R.; Lopez, R.; De Frutos, M.; Garcia, E.
    First molecular characterization of a uridine diphosphate galacturonate 4-epimerase: an enzyme required for capsular biosynthesis in Streptococcus pneumoniae type 1 (1999), Mol. Microbiol., 31, 703-713.
    View publication on PubMed
Search for more literature for 5.1.3.6

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
CDP-glucose
-
 Streptococcus pneumoniae
galactose
-
 Streptococcus pneumoniae
GDP-glucose
-
 Streptococcus pneumoniae
GMP
-
 Streptococcus pneumoniae
NaCl progressive decrease in activity above 100 mM NaCl, activity drops below 50% at 250 mM Streptococcus pneumoniae
p-hydroxymercuribenzoate reactivation by NAD+ and 2-mercaptoethanol Streptococcus pneumoniae
UDP
-
 Streptococcus pneumoniae
UDP-galactose
-
 Streptococcus pneumoniae
UDP-glucose
-
 Streptococcus pneumoniae
UDP-mannose
-
 Streptococcus pneumoniae
UDP-xylose
-
 Streptococcus pneumoniae
UMP
-
 Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
 UDP-D-glucuronate pH 7.5, 37°C Streptococcus pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 2 * 40000, SDS-PAGE Streptococcus pneumoniae
80000
-
 gel filtration Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Streptococcus pneumoniae the enzyme is required for capsular biosynthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
 type 1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is required for capsular biosynthesis Streptococcus pneumoniae ?
-
 ?
UDP-D-glucuronate equilibrium constant in direction of UDP-galacturonate formation is 1.3 Streptococcus pneumoniae UDP-D-galacturonate
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Streptococcus pneumoniae

Synonyms

Synonyms Comment Organism
UDPGLE
-
 Streptococcus pneumoniae
uridine diphosphate galacturonate 4-epimerase
-
 Streptococcus pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 42
-
 Streptococcus pneumoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 60 min, about 35% loss of activity Streptococcus pneumoniae
30
-
 60 min, about 50% loss of activity Streptococcus pneumoniae
37
-
 60 min, about 75% loss of activity Streptococcus pneumoniae
42
-
 60 min, about 20% loss of activity Streptococcus pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Streptococcus pneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10 pH 6.5: about 50% of maximal activity, pH 10.0: about 25% of maximal activity Streptococcus pneumoniae

Cofactor

Cofactor Comment Organism Structure
NAD+ 50 nM to 5 mM, does not affect activity Streptococcus pneumoniae