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Literature summary for 5.1.3.6 extracted from
- Enzymatic characterization and comparison of various poaceae UDP-GlcA 4-epimerase isoforms (2009), J. Biochem., 146, 527-534.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Arabidopsis thaliana |
| expression in Escherichia coli | Oryza sativa |
| recombinant ZmUGlcAE, lacking the putative transmembrane domain at amino acid position 1-47, is expressed in Escherichia coli | Zea mays |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| DELTA1-47 | recombinant product ZmUGlcAE, lacking the putative transmembrane domain, converts UDP-glucuronic acid to UDP-alpha-D-galacturonic acid while control Escherichia coli cells containing empty vector have no enzyme activity | Zea mays |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| UDP | - |
Arabidopsis thaliana |  |
| UDP | - |
Oryza sativa | |
| UDP | 2 mM, strong inhibition of recombinant ZmUGlcAE1-47 | Zea mays | |
| UDP-xylose | - |
Arabidopsis thaliana | |
| UDP-xylose | - |
Oryza sativa | |
| UDP-xylose | 2 mM, strong inhibition of recombinant ZmUGlcAE1-47 | Zea mays | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.259 | - |
UDP-glucuronate | recombinant OsUGlcAE3, lacking the amino acid position 1-53, in 0.1 M sodium phosphate, pH 7.6 | Oryza sativa | |
| 0.358 | - |
UDP-glucuronate | recombinant AtUGlcAE2, lacking the amino acid position 1-68, in 0.1 M sodium phosphate, pH 7.6 | Arabidopsis thaliana | |
| 0.397 | - |
UDP-glucuronate | recombinant ZmUGlcAE, lacking the putative transmembrane domain at amino acid position 1-47, in 0.1 M sodium phosphate, pH 7.6 | Zea mays | |
| 0.426 | - |
UDP-glucuronate | recombinant AtUGlcAE3, lacking the amino acid position 1-53, in 0.1 M sodium phosphate, pH 7.6 | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42900 | - |
2 * 42900, recombinant ZmUGlcAE, lacking the putative transmembrane domain at amino acid position 1-47, determined by gel filtration | Zea mays |
| 44100 | - |
2 * 44100, recombinant enzyme, lacking the putative transmembrane domain at amino acid position 1-53, determined by gel filtration | Arabidopsis thaliana |
| 44300 | - |
2 * 44300, recombinant OsUGlcAE3, lacking the putative transmembrane domain at amino acid position 1-53, determined by gel filtration | Oryza sativa |
| 45500 | - |
2 * 45500, recombinant AtUGlcAE2, lacking the putative transmembrane domain at amino acid position 1-68, determined by gel filtration | Arabidopsis thaliana |
| 81400 | - |
recombinant ZmUGlcAE, lacking the putative transmembrane domain at amino acid position 1-47, determined by gel filtration | Zea mays |
| 88500 | - |
recombinant AtUGlcAE2, lacking the putative transmembrane domain at amino acid position 1-68, determined by gel filtration | Arabidopsis thaliana |
| 88500 | - |
recombinant AtUGlcAE3, lacking the putative transmembrane domain at amino acid position 1-53, determined by gel filtration | Arabidopsis thaliana |
| 92200 | - |
recombinant OsUGlcAE3, lacking the putative transmembrane domain at amino acid position 1-53, determined by gel filtration | Oryza sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucuronate | Arabidopsis thaliana | - |
UDP-D-galacturonate | - |
r | |
| UDP-glucuronate | Zea mays | - |
UDP-D-galacturonate | - |
r | |
| UDP-glucuronate | Oryza sativa | - |
UDP-D-galacturonate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | O22141 | - |
- |
| Arabidopsis thaliana | Q9LIS3 | - |
- |
| Arabidopsis thaliana | Q9M0B6 | - |
- |
| Oryza sativa | Q6K9M5 | - |
- |
| Zea mays | Q304Y2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by using gel filtration and anion exchange chromatography | Arabidopsis thaliana |
| by using gel filtration and anion exchange chromatography | Zea mays |
| by using gel filtration and anion exchange chromatography | Oryza sativa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, recombinant enzyme ZmUGlcAE lacking the putative transmembrane domain is stable (more than 6 months) when stored as a crude extract | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucuronate | - |
Arabidopsis thaliana | UDP-D-galacturonate | - |
r | |
| UDP-glucuronate | - |
Zea mays | UDP-D-galacturonate | - |
r | |
| UDP-glucuronate | - |
Oryza sativa | UDP-D-galacturonate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42900, recombinant ZmUGlcAE, lacking the putative transmembrane domain at amino acid position 1-47, determined by gel filtration | Zea mays |
| dimer | 2 * 44100, recombinant enzyme, lacking the putative transmembrane domain at amino acid position 1-53, determined by gel filtration | Arabidopsis thaliana |
| dimer | 2 * 44300, recombinant OsUGlcAE3, lacking the putative transmembrane domain at amino acid position 1-53, determined by gel filtration | Oryza sativa |
| dimer | 2 * 45500, recombinant AtUGlcAE2, lacking the putative transmembrane domain at amino acid position 1-68, determined by gel filtration | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtUGlcAE1 | - |
Arabidopsis thaliana |
| AtUGlcAE2 | - |
Arabidopsis thaliana |
| AtUGlcAE3 | - |
Arabidopsis thaliana |
| OsUGlcAE3 | - |
Oryza sativa |
| UDP-GlcA 4-epimerase | - |
Zea mays |
| UDP-GlcA 4-epimerase1 | - |
Arabidopsis thaliana |
| UDP-GlcA 4-epimerase2 | - |
Arabidopsis thaliana |
| UDP-GlcA 4-epimerase3 | - |
Arabidopsis thaliana |
| UDP-GlcA 4-epimerase3 | - |
Oryza sativa |
| UGlcAE | - |
Zea mays |
| ZmUGlcAE | - |
Zea mays |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Arabidopsis thaliana |
| 37 | - |
assay at | Zea mays |
| 37 | - |
recombinant AtUGlcAE2, lacking the amino acid position 1-68 | Arabidopsis thaliana |
| 37 | - |
recombinant OsUGlcAE3, lacking the amino acid position 1-53 | Oryza sativa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| -20 | - |
recombinant enzyme is still fully active after 1 year in storage | Arabidopsis thaliana |
| -20 | - |
recombinant enzyme ZmUGlcAE lacking the putative transmembrane domain is stable (more than 6 months) when stored as a crude extract at 20°C | Zea mays |
| -20 | 65 | the activity of ZmUGlcAE lacking the putative transmembrane domain is completely abolished when assays are conducted at pH value below 4 or above 10, or when the assay temperature is above 65°C | Zea mays |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
UDP-glucuronate | recombinant AtUGlcAE2, lacking the amino acid position 1-68, in 0.1 M sodium phosphate, pH 7.6 | Arabidopsis thaliana | |
| 10 | - |
UDP-glucuronate | recombinant AtUGlcAE3, lacking the amino acid position 1-53, in 0.1 M sodium phosphate, pH 7.6 | Arabidopsis thaliana | |
| 10.1 | - |
UDP-glucuronate | recombinant ZmUGlcAE, lacking the putative transmembrane domain at amino acid position 1-47, in 0.1 M sodium phosphate, pH 7.6 | Zea mays | |
| 37.4 | - |
UDP-glucuronate | recombinant OsUGlcAE3, lacking the amino acid position 1-53, in 0.1 M sodium phosphate, pH 7.6 | Oryza sativa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 7.8 | optimal pH in phosphate buffer for the activity of recombinant enzyme ZmUGlcAE1-47 lacking the transmembrane domain | Zea mays |
| 7.6 | - |
recombinant AtUGlcAE2, lacking the amino acid position 1-68 | Arabidopsis thaliana |
| 7.6 | - |
recombinant AtUGlcAE3, lacking the amino acid position 1-53 | Arabidopsis thaliana |
| 7.6 | - |
recombinant OsUGlcAE3, lacking the amino acid position 1-53 | Oryza sativa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | the activity of ZmUGlcAE1-47 is completely abolished when assays are conducted at pH value below 4 or above 10 | Zea mays |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0632 | - |
UDP | recombinant ZmUGlcAE, position 1-47 is lacking | Zea mays | |
| 0.0731 | - |
UDP-xylose | recombinant ZmUGlcAE, position 1-47 is lacking | Zea mays | |
| 0.1107 | - |
UDP | recombinant AtUGlcAE3, position 1-53 is lacking | Arabidopsis thaliana | |
| 0.1145 | - |
UDP | recombinant OsUGlcAE3, position 1-53 is lacking | Oryza sativa | |
| 0.1341 | - |
UDP | recombinant AtUGlcAE2, position 1-68 is lacking | Arabidopsis thaliana | |
| 0.1436 | - |
UDP | recombinant AtUGlcAE1, position 1-64 is lacking | Arabidopsis thaliana | |
| 0.1599 | - |
UDP-xylose | recombinant OsUGlcAE3, position 1-53 is lacking | Oryza sativa | |
| 0.2473 | - |
UDP-xylose | recombinant AtUGlcAE3, position 1-53 is lacking | Arabidopsis thaliana | |
| 0.2972 | - |
UDP-xylose | recombinant AtUGlcAE1, position 1-64 is lacking | Arabidopsis thaliana | |
| 0.3051 | - |
UDP-xylose | recombinant AtUGlcAE2, position 1-68 is lacking | Arabidopsis thaliana | |
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