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5.1.1.17: isopenicillin-N epimerase

This is an abbreviated version!
For detailed information about isopenicillin-N epimerase, go to the full flat file.

Word Map on EC 5.1.1.17

Reaction

isopenicillin N
=
Penicillin N

Synonyms

cefD2, IPN epimerase, isopenecillin N-CoA epimerase, isopenicillin N epimerase, isopenicillin N-CoA epimerase, PenN epimerase

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.1 Acting on amino acids and derivatives
                5.1.1.17 isopenicillin-N epimerase

Purification

Purification on EC 5.1.1.17 - isopenicillin-N epimerase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DE-52, DEAE-Affi-gel blue, Sephadex G-200, calcium phosphate, Mono Q
-
ammonium sulfate, Sephadex G-200, DEAE-trisacryl
-
by anion exchange chromatography, purified IPN epimerase is able to act in both orientations, leading in vitro to an equimolecular mixture of isopenicillin N and penicillin N
-
protamine sulfate, ammonium sulfate, Sephadex G-75, Mono Q
-