5.1.1.17: isopenicillin-N epimerase

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For detailed information about isopenicillin-N epimerase, go to the full flat file.

Word Map on EC 5.1.1.17

5.1.1.17

cephalosporin

acremonium

chrysogenum

beta-lactam

clavuligerus

epimerization

deacetoxycephalosporin

cefef

pyridoxal

pcbab

deacetylcephalosporin

cephamycins

expandase

acyltransferase

pende

untransformed

alpha-aminoadipic

lactamdurans

daocs

biotechnology

synthesis

medicine

Reaction

Synonyms

cefD2, IPN epimerase, isopenecillin N-CoA epimerase, isopenicillin N epimerase, isopenicillin N-CoA epimerase, PenN epimerase

ECTree

5 Isomerases

5.1 Racemases and epimerases

5.1.1 Acting on amino acids and derivatives

5.1.1.17 isopenicillin-N epimerase

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Results	in table
500	AA Sequence
5	Application
1	CAS Registry Number
3	Cloned(Commentary)
5	Cofactor
2	Expression
2	General Stability
21	Inhibitors
6	KM Value [mM]
6	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
20	Organism
4	Pathway
3	pH Optimum
1	pH Range
2	pH Stability
4	Purification (Commentary)
2	Reaction
5	Reaction Type
17	Reference
2	Source Tissue
7	Specific Activity [micromol/min/mg]
2	Storage Stability
28	Substrates and Products (Substrate)
3	Subunits
13	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Temperature Stability [°C]

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Cloned on EC 5.1.1.17 - isopenicillin-N epimerase

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expression in Penicillium chrysogenum

2 entries

Penecillium chrysogenum strains are constructed expressing cefD1 (isopenecillin N-CoA synthetase), cefD2 (isopenecillin N-CoA epimerase), cefEF (deacetylcephalosporin-actyltransferase), and cefG (deacetylcephalosporin C synthetase) genes cloned from Acremonium chrysogenum. HPLC analysis of cell extracts show that transformant TA64, TA71, and TA98 accumulate intracellularly deacetylcephalosporin C and, in the last strain (TA98), also cephalosporin C.

Acremonium chrysogenum

673028