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4.5.1.B1: hexachlorocyclohexane dehydrochlorinase

This is an abbreviated version!
For detailed information about hexachlorocyclohexane dehydrochlorinase, go to the full flat file.

Word Map on EC 4.5.1.B1

Reaction

1,3,4,5,6-pentachlorocyclohexene
=
1,3,4,6-tetrachlorocyclohexa-1,4-diene
 +
HCl

Synonyms

dehydrochlorinase, gamma-HCH dehydrochlorinase, gamma-hexachlorocyclohexane dehydrochlorinase, HCH dehydrochlorinase, LinA, LinA-type1, LinA-type2, LinA-UT26, Lina1, Lina2

ECTree

     4 Lyases
         4.5 Carbon-halide lyases
             4.5.1 Carbon-halide lyases (only sub-subclass identified to date)
                4.5.1.B1 hexachlorocyclohexane dehydrochlorinase

Cloned

Cloned on EC 4.5.1.B1 - hexachlorocyclohexane dehydrochlorinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta (DE3) cells
-
expressed in Pseudomonas nitroreducens strain J5-1
gene linA, DNA and amino acid sequence and genetic structure analysis
D4Z258, P59767, Q6VQZ2
gene linA, DNA and amino acid sequence determination and analysis, genetic structure analysis
gene linA, DNA and amino acid sequence determination and analysis, sequence comparison, subcloning in Escherichia coli strain DH5alpha, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene linA-UT26, sequence comparison