Information on EC 4.5.1.B1 - hexachlorocyclohexane dehydrochlorinase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.5.1.B1
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
hexachlorocyclohexane dehydrochlorinase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1,3,4,5,6-pentachlorocyclohexene = 1,3,4,6-tetrachlorocyclohexa-1,4-diene + HCl
show the reaction diagram
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hexachlorocyclohexane = 1,3,4,5,6-pentachlorocyclohexene + HCl
show the reaction diagram
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene linA
UniProt
Manually annotated by BRENDA team
gene linA
UniProt
Manually annotated by BRENDA team
gene linA
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
changed residues K20Q, L96C and A131G, vicinal to the active site, are probably responsible for the altered enantioselectivity of LinA-type2 compared to LinA-type1, structural comparison of LinA-type2 and -type1 proteins suggests that thermostability of LinA-type2 might partly arise due to presence of higher number of ionic interactions, along with 4% increase in the intersubunit buried surface area; changed residues K20Q, L96C and A131G, vicinal to the active site, are probably responsible for the altered enantioselectivity of LinA-type2 compared to LinA-type1, structural comparison of LinA-type2 and -type1 proteins suggests that thermostability of LinA-type2 might partly arise due to presence of higher number of ionic interactions, along with 4% increase in the intersubunit buried surface area
metabolism
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,3,4,5,6-pentachlorocyclohexene
1,3,4,6-tetrachloro-1,4-cyclohexadiene + HCl
show the reaction diagram
alpha-hexachlorocyclohexane
beta-pentachlorocyclohexene + HCl
show the reaction diagram
delta-hexachlorocyclohexane
1,3,4,6-tetrachloro-1,4-cyclohexadiene + 2 HCl
show the reaction diagram
delta-pentachlorocyclohexene
1,3,4,6-tetrachloro-1,4-cyclohexadiene + HCl
show the reaction diagram
gamma-hexachlorocyclohexane
1,3,4,6-tetrachloro-1,4-cyclohexadiene + 2 HCl
show the reaction diagram
gamma-hexachlorocyclohexane
gamma-pentachlorocyclohexene + HCl
show the reaction diagram
gamma-hexachlorocyclohexane
pentachlorocyclohexene + HCl
show the reaction diagram
gamma-pentachlorocyclohexene
1,3,4,6-tetrachloro-1,4-cyclohexadiene + HCl
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1,3,4,5,6-pentachlorocyclohexene
1,3,4,6-tetrachloro-1,4-cyclohexadiene + HCl
show the reaction diagram
B5ANU3, B5ANV6
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-
-
?
gamma-hexachlorocyclohexane
pentachlorocyclohexene + HCl
show the reaction diagram
B5ANU3, B5ANV6
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-
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 65
activity range, rapid drop in activity above 60°C, profile overview
30 - 75
activity range, rapid drop in activity above 70°C, profile overview
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 16500, SDS-PAGE
homotetramer
homotrimer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
using 100 mM HEPES-Na (pH 7.0), 16% (w/v) polyethylene glycol 3350, and 200 mM ammonium acetate
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
Tm, complete inactivation after 1 h
45 - 65
8 h, completely stable, Tm is 65°C
45
loss of 50% activity after 60 min
additional information
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration; recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta (DE3) cells
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expressed in Pseudomonas nitroreducens strain J5-1
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gene linA, DNA and amino acid sequence and genetic structure analysis
gene linA, DNA and amino acid sequence and genetic structure analysis; gene linA, DNA and amino acid sequence and genetic structure analysis
gene linA, DNA and amino acid sequence determination and analysis, genetic structure analysis
gene linA, DNA and amino acid sequence determination and analysis, sequence comparison, subcloning in Escherichia coli strain DH5alpha, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3); gene linA, DNA and amino acid sequence determination and analysis, sequence comparison, subcloning in Escherichia coli strain DH5alpha, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene linA-UT26, sequence comparison