4.4.1.29: phycobiliprotein cysteine-84 phycobilin lyase
This is an abbreviated version!
For detailed information about phycobiliprotein cysteine-84 phycobilin lyase, go to the full flat file.
Word Map on EC 4.4.1.29
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4.4.1.29
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phycoerythrocyanin
-
chromophore
-
allophycocyanin
-
phycobiliproteins
-
phycocyanin
-
biliproteins
-
apoproteins
-
lyases
-
beta-subunits
-
phycoerythrobilin
-
phycobilisomes
-
light-harvesting
-
synechococcus
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cyanobacterial
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regioselective
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chromophorylated
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maxima
-
anabaena
-
nostoc
-
s-type
-
imidazole
-
synthesis
- 4.4.1.29
-
phycoerythrocyanin
- chromophore
-
allophycocyanin
-
phycobiliproteins
- phycocyanin
-
biliproteins
- apoproteins
- lyases
- beta-subunits
- phycoerythrobilin
-
phycobilisomes
-
light-harvesting
- synechococcus
-
cyanobacterial
-
regioselective
-
chromophorylated
-
maxima
- anabaena
- nostoc
-
s-type
- imidazole
- synthesis
Reaction
Synonyms
alr0617, CpcS-1, CpcS-III, CpcS1, CpcSU lyase, CpcU, cpeS, CpeS1, PCB:Cys-beta84-phycobiliprotein lyase, phycobilin:cystein-84 biliprotein lyase, phycocyanobilin:Cys-beta84-phycobiliprotein lyase
ECTree
4.4.1.29 phycobiliprotein cysteine-84 phycobilin lyaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 4.4.1.29 - phycobiliprotein cysteine-84 phycobilin lyase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
apo allophycocyanin alpha-subunit + (2R,3E)-phycocyanobilin
[allophycocyanin alpha-subunit]-Cys81-phycocyanobilin
apo allophycocyanin beta-subunit + (2R,3E)-phycocyanobilin
[allophycocyanin beta-subunit]-Cys81-phycocyanobilin
apo C-phycocyanin beta-subunit + (2R,3E)-phycocyanobilin
[C-phycocyanin beta-subunit]-Cys82-phycocyanobilin
apo-allophycocyanin beta-subunit + (2R,3E)-phycocyanobilin
[allophycocyanin beta-subunit]-Cys81-phycocyanobilin
apo-phycoerythrocyanin beta-subunit + (2R,3E)-phycocyanobilin
[phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
apo-[allophycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin
[allophycocyanin alpha-subunit]-Cys81-phycocyanobilin
apo-[allophycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin
[allophycocyanin alpha-subunit]-Cys84-phycocyanobilin
apo-[allophycocyanin alpha-subunit] + phycoerythrobilin
[allophycocyanin alpha-subunit]-Cys84-phycoerythrobilin
Thermosynechococcus vestitus
-
-
-
?
apo-[allophycocyanin alpha-subunit] + phytochromobilin
[allophycocyanin alpha-subunit]-Cys84-phytochromobilin
Thermosynechococcus vestitus
-
-
-
?
apo-[allophycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[allophycocyanin beta-subunit]-Cys84-phycocyanobilin
apo-[allophycocyanin beta-subunit] + phycoerythrobilin
[allophycocyanin beta-subunit]-Cys84-phycoerythrobilin
Thermosynechococcus vestitus
-
-
-
?
apo-[allophycocyanin beta-subunit] + phytochromobilin
[allophycocyanin beta-subunit]-Cys84-phytochromobilin
Thermosynechococcus vestitus
-
-
-
?
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[C-phycocyanin beta-subunit]-Cys82-phycocyanobilin
-
-
-
?
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
apo-[C-phycoerythrin alpha-subunit] + (3E)-phycoerythrobilin
[C-phycoerythrin alpha-subunit]-Cys84-phycoerythrobilin
-
-
-
?
apo-[C-phycoerythrin beta-subunit] + (2R,3E)-phycoerythrobilin
[C-phycoerythrin beta-subunit]-Cys82-phycoerythrobilin
apo-[C-phycoerythrin beta-subunit] + (3E)-phycoerythrobilin
[C-phycoerythrin beta-subunit]-Cys84-phycoerythrobilin
-
-
-
?
apo-[phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[phycocyanin beta-subunit]-Cys82-phycocyanobilin
Thermosynechococcus vestitus
-
-
-
?
apo-[phycocyanin beta-subunit] + phycoerythrobilin
[phycocyanin beta-subunit]-Cys82-phycoerythrobilin
Thermosynechococcus vestitus
-
-
-
?
apo-[phycocyanin beta-subunit] + phytochromobilin
[phycocyanin beta-subunit]-Cys82-phytochromobilin
Thermosynechococcus vestitus
-
-
-
?
apo-[phycoerythrin alpha-subunit] + phycoerythrobilin
[phycoerythrin alpha-subunit]-Cys82-phycoerythrobilin
apo-[phycoerythrin beta-subunit] + 3Z-phycoerythrobilin
[phycoerythrin beta-subunit]-Cys82-phycoerythrobilin
apo-[phycoerythrin beta-subunit] + phycocyanobilin
[phycoerythrin beta-subunit]-Cys82-phycocyanobilin
apo-[phycoerythrin beta-subunit] + phycoerythrobilin
[phycoerythrin beta-subunit]-Cys80-phycoerythrobilin
-
CpeS bilin lyase efficiently ligates phycoerythrobilin to Cys80 of phycoerythrin beta-subunit
-
?
apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
apo allophycocyanin alpha-subunit + (2R,3E)-phycocyanobilin
[allophycocyanin alpha-subunit]-Cys81-phycocyanobilin
the enzyme is involved in phycobiliprotein biogenesis
-
-
?
apo allophycocyanin alpha-subunit + (2R,3E)-phycocyanobilin
[allophycocyanin alpha-subunit]-Cys81-phycocyanobilin
the enzyme is involved in phycobiliprotein biogenesis
-
-
?
apo allophycocyanin beta-subunit + (2R,3E)-phycocyanobilin
[allophycocyanin beta-subunit]-Cys81-phycocyanobilin
the enzyme is involved in phycobiliprotein biogenesis
-
-
?
apo allophycocyanin beta-subunit + (2R,3E)-phycocyanobilin
[allophycocyanin beta-subunit]-Cys81-phycocyanobilin
the enzyme is involved in phycobiliprotein biogenesis
-
-
?
apo C-phycocyanin beta-subunit + (2R,3E)-phycocyanobilin
[C-phycocyanin beta-subunit]-Cys82-phycocyanobilin
the enzyme is involved in phycobiliprotein biogenesis
-
-
?
apo C-phycocyanin beta-subunit + (2R,3E)-phycocyanobilin
[C-phycocyanin beta-subunit]-Cys82-phycocyanobilin
the enzyme is involved in phycobiliprotein biogenesis
-
-
?
apo-allophycocyanin beta-subunit + (2R,3E)-phycocyanobilin
[allophycocyanin beta-subunit]-Cys81-phycocyanobilin
-
-
-
?
apo-allophycocyanin beta-subunit + (2R,3E)-phycocyanobilin
[allophycocyanin beta-subunit]-Cys81-phycocyanobilin
-
-
-
?
apo-phycoerythrocyanin beta-subunit + (2R,3E)-phycocyanobilin
[phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
-
-
-
?
apo-phycoerythrocyanin beta-subunit + (2R,3E)-phycocyanobilin
[phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
-
-
-
?
apo-[allophycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin
[allophycocyanin alpha-subunit]-Cys81-phycocyanobilin
-
-
-
?
apo-[allophycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin
[allophycocyanin alpha-subunit]-Cys81-phycocyanobilin
-
-
-
?
apo-[allophycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin
[allophycocyanin alpha-subunit]-Cys84-phycocyanobilin
-
-
-
?
apo-[allophycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin
[allophycocyanin alpha-subunit]-Cys84-phycocyanobilin
-
-
-
?
apo-[allophycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin
[allophycocyanin alpha-subunit]-Cys84-phycocyanobilin
-
both subunits CpcS-1 and CpcU are required to attach phycocyanobilin to allophycocyanin subunits ApcD (alpha) and ApcF (beta)
-
?
apo-[allophycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin
[allophycocyanin alpha-subunit]-Cys84-phycocyanobilin
Thermosynechococcus vestitus
-
-
-
?
apo-[allophycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[allophycocyanin beta-subunit]-Cys84-phycocyanobilin
-
-
-
?
apo-[allophycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[allophycocyanin beta-subunit]-Cys84-phycocyanobilin
-
-
-
?
apo-[allophycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[allophycocyanin beta-subunit]-Cys84-phycocyanobilin
-
both subunits CpcS-1 and CpcU are required to attach phycocyanobilin to allophycocyanin subunits ApcD (alpha) and ApcF (beta)
-
?
apo-[allophycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[allophycocyanin beta-subunit]-Cys84-phycocyanobilin
Thermosynechococcus vestitus
-
-
-
?
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
-
-
-
?
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins
-
-
?
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
the enzyme attaches (2R,3E)-phycocyanobilin to native apo C-phycocyanin beta-subunit and to the C155I-mutant, but not to theC84S mutant
-
-
?
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins
-
-
?
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
the enzyme attaches (2R,3E)-phycocyanobilin to native apo C-phycocyanin beta-subunit and to the C155I-mutant, but not to theC84S mutant
-
-
?
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
-
-
-
?
apo-[C-phycoerythrin beta-subunit] + (2R,3E)-phycoerythrobilin
[C-phycoerythrin beta-subunit]-Cys82-phycoerythrobilin
-
-
-
-
?
apo-[C-phycoerythrin beta-subunit] + (2R,3E)-phycoerythrobilin
[C-phycoerythrin beta-subunit]-Cys82-phycoerythrobilin
-
-
-
-
?
apo-[phycoerythrin alpha-subunit] + phycoerythrobilin
[phycoerythrin alpha-subunit]-Cys82-phycoerythrobilin
-
CpeS bilin lyase ligates phycoerythrobilin at both Cys82 and Cys139 of phycoerythrin alpha-subunit, but very inefficiently
-
?
apo-[phycoerythrin alpha-subunit] + phycoerythrobilin
[phycoerythrin alpha-subunit]-Cys82-phycoerythrobilin
-
ligase subunit CpeY alone, but not subunit CpeZ, can ligate phycoerythrobilin to phycoerythrin alpha-subunit, but the yield of phycoerythrin alpha-subunit-phycoerythrobilin is lower than achieved with subunits CpeY and CpeZ together
-
?
apo-[phycoerythrin beta-subunit] + 3Z-phycoerythrobilin
[phycoerythrin beta-subunit]-Cys82-phycoerythrobilin
-
isoform CpeS is specific for 3Z-phycoerythrobilin transfer to Cys82 of phycoerythrin and essential for the correct configuration of the attachment product
-
?
apo-[phycoerythrin beta-subunit] + 3Z-phycoerythrobilin
[phycoerythrin beta-subunit]-Cys82-phycoerythrobilin
-
isoform CpeS is specific for 3Z-phycoerythrobilin transfer to Cys82 of phycoerythrin and essential for the correct configuration of the attachment product
-
?
apo-[phycoerythrin beta-subunit] + phycocyanobilin
[phycoerythrin beta-subunit]-Cys82-phycocyanobilin
-
-
-
?
apo-[phycoerythrin beta-subunit] + phycocyanobilin
[phycoerythrin beta-subunit]-Cys82-phycocyanobilin
-
-
-
?
apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins
-
-
?
apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
the enzyme attaches (2R,3E)-phycocyanobilin to native apo phycoerythrocyanin beta-subunit and to the C155I-mutant, but not to theC84S mutant
-
-
?
apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins
-
-
?
apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
the enzyme attaches (2R,3E)-phycocyanobilin to native apo phycoerythrocyanin beta-subunit and to the C155I-mutant, but not to theC84S mutant
-
-
?
additional information
?
-
the enzyme shows no activity with the alpha-subunits from phycocyanin and phycoerythrocyanin
-
-
?
additional information
?
-
the enzyme shows no activity with the alpha-subunits from phycocyanin and phycoerythrocyanin
-
-
?
additional information
?
-
-
enzyme catalyses the attachment of phycobilin chromophores to cysteine 82 of the beta-subunit of Cpc
-
-
?
additional information
?
-
-
enzyme catalyses the attachment of phycobilin chromophores to cysteine 82 of the beta-subunit of Cpc
-
-
?
additional information
?
-
-
neither subunit CpeY, subunit CpeZ, nor CpeY/CpeZ can ligate phycoerythrobilin to phycoerythrin beta-subunit
-
-
?
additional information
?
-
neither subunit CpeY, subunit CpeZ, nor CpeY/CpeZ can ligate phycoerythrobilin to phycoerythrin beta-subunit
-
-
?
additional information
?
-
in vitro, enzyme forms complexes with phycocyanobilin lyase CpcT which attaches phycocyanobilin to Cys155 of C-phycocyanin subunit beta. Attachment of a chromophore at cysteine84 inhibits the subsequent attachment to cysteine 155, whereas the inverse sequence generates subunits carrying both chromophores
-
-
?
additional information
?
-
-
in vitro, enzyme forms complexes with phycocyanobilin lyase CpcT which attaches phycocyanobilin to Cys155 of C-phycocyanin subunit beta. Attachment of a chromophore at cysteine84 inhibits the subsequent attachment to cysteine 155, whereas the inverse sequence generates subunits carrying both chromophores
-
-
?
additional information
?
-
isoform CpeS1 can bind phycocyanobilin. Binding is rapid, and the chromophore is bound in an extended conformation similar to that in phycobiliproteins but only poorly fluorescent. Upon addition of apo-biliproteins, the chromophore is transferred to the latter much slower, indicating that chromophorylated CpeS1 is an intermediate in the enzymatic reaction. In addition, imidazole is bound to phycocyanobilin
-
-
?
additional information
?
-
in vitro, enzyme forms complexes with phycocyanobilin lyase CpcT which attaches phycocyanobilin to Cys155 of C-phycocyanin subunit beta. Attachment of a chromophore at cysteine84 inhibits the subsequent attachment to cysteine 155, whereas the inverse sequence generates subunits carrying both chromophores
-
-
?
additional information
?
-
isoform CpeS1 can bind phycocyanobilin. Binding is rapid, and the chromophore is bound in an extended conformation similar to that in phycobiliproteins but only poorly fluorescent. Upon addition of apo-biliproteins, the chromophore is transferred to the latter much slower, indicating that chromophorylated CpeS1 is an intermediate in the enzymatic reaction. In addition, imidazole is bound to phycocyanobilin
-
-
?
additional information
?
-
dimeric isoform CpeS is able to bind two molecules of precursor 15,16-dihydrobiliverdin, 3E-phycoerythrobilin, or 3Z-phycoerythrobilin with similar binding kinetics and high affinity. CpeS dimer formation occurs prior to bilin binding. CpeS transfers phycoerythrobilin but not 15,16-dihydrobiliverdin to dimeric phycoerythrin. Only CpeS 3Z-phycoerythrobilin-mediated reactions result in formation of holo-phycoerythrin with identical spectroscopic properties as native-phycoerythrin
-
-
?
additional information
?
-
dimeric isoform CpeS is able to bind two molecules of precursor 15,16-dihydrobiliverdin, 3E-phycoerythrobilin, or 3Z-phycoerythrobilin with similar binding kinetics and high affinity. CpeS dimer formation occurs prior to bilin binding. CpeS transfers phycoerythrobilin but not 15,16-dihydrobiliverdin to dimeric phycoerythrin. Only CpeS 3Z-phycoerythrobilin-mediated reactions result in formation of holo-phycoerythrin with identical spectroscopic properties as native-phycoerythrin
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-
?
