Information on EC 4.4.1.29 - phycobiliprotein cysteine-84 phycobilin lyase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.4.1.29
-
RECOMMENDED NAME
GeneOntology No.
phycobiliprotein cysteine-84 phycobilin lyase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[allophycocyanin alpha-subunit]-Cys84-phycocyanobilin = apo-[allophycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin
show the reaction diagram
[allophycocyanin beta-subunit]-Cys84-phycocyanobilin = apo-[allophycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
show the reaction diagram
[C-phycocyanin beta-subunit]-Cys84-phycocyanobilin = apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
show the reaction diagram
[C-phycoerythrin alpha-subunit]-Cys84-phycoerythrobilin = apo-[C-phycoerythrin alpha-subunit] + (2R,3E)-phycoerythrobilin
show the reaction diagram
[C-phycoerythrin beta-subunit]-Cys84-phycoerythrobilin = apo-[C-phycoerythrin beta-subunit] + (2R,3E)-phycoerythrobilin
show the reaction diagram
[phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin = apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
[phycobiliprotein]-Cys84-phycobilin:phycobilin lyase
The enzyme, found in cyanobacteria and red algae, catalyses the attachment of phycobilin chromophores to cysteine 84 of several phycobiliproteins (the numbering used here corresponds to the enzyme from Anabaena, in other organisms the number may vary slightly). It can attach phycocyanobilin to the beta subunits of C-phycocyanin and phycoerythrocyanin and to both subunits of allophycocyanin. In addition, it can attach phycoerythrobilin to both subunits of C-phycoerythrin.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
sSingle and double mutant strains for lyase subunits cpcSI and cpcU exhibit slower growth rates, reduced apo-phycobiliprotein levels, and impaired assembly of phycobilisomes. A cpcSI cpcU cpcT triple mutant is nearly devoid of apo-phycobiliprotein. The cpcSI and cpcU mutants produce an altered form of the phycocyanin beta-subunit, which has a mass about 588 Da smaller than the wild-type protein. The modified phycobilisomes from the cpcsI, cpcU, and cpcSIcpcU mutant strains carry a phycocyanobilin chromophore at Cys153 of CpcB but not at Cys82. Both CpcSI and CpcU are required for covalent attachment of phycocyanobilin to Cys82 of the phycocyanin subunit
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
apo allophycocyanin alpha-subunit + (2R,3E)-phycocyanobilin
[allophycocyanin alpha-subunit]-Cys81-phycocyanobilin
show the reaction diagram
apo allophycocyanin beta-subunit + (2R,3E)-phycocyanobilin
[allophycocyanin beta-subunit]-Cys81-phycocyanobilin
show the reaction diagram
apo C-phycocyanin beta-subunit + (2R,3E)-phycocyanobilin
[C-phycocyanin beta-subunit]-Cys82-phycocyanobilin
show the reaction diagram
apo-allophycocyanin beta-subunit + (2R,3E)-phycocyanobilin
[allophycocyanin beta-subunit]-Cys81-phycocyanobilin
show the reaction diagram
apo-phycoerythrocyanin beta-subunit + (2R,3E)-phycocyanobilin
[phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
show the reaction diagram
apo-[allophycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin
[allophycocyanin alpha-subunit]-Cys81-phycocyanobilin
show the reaction diagram
apo-[allophycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin
[allophycocyanin alpha-subunit]-Cys84-phycocyanobilin
show the reaction diagram
apo-[allophycocyanin alpha-subunit] + phycoerythrobilin
[allophycocyanin alpha-subunit]-Cys84-phycoerythrobilin
show the reaction diagram
-
-
-
?
apo-[allophycocyanin alpha-subunit] + phytochromobilin
[allophycocyanin alpha-subunit]-Cys84-phytochromobilin
show the reaction diagram
-
-
-
?
apo-[allophycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[allophycocyanin beta-subunit]-Cys84-phycocyanobilin
show the reaction diagram
apo-[allophycocyanin beta-subunit] + phycoerythrobilin
[allophycocyanin beta-subunit]-Cys84-phycoerythrobilin
show the reaction diagram
-
-
-
?
apo-[allophycocyanin beta-subunit] + phytochromobilin
[allophycocyanin beta-subunit]-Cys84-phytochromobilin
show the reaction diagram
-
-
-
?
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[C-phycocyanin beta-subunit]-Cys82-phycocyanobilin
show the reaction diagram
A8HTN2 and A8HTM2
-
-
-
?
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
show the reaction diagram
apo-[C-phycoerythrin alpha-subunit] + (3E)-phycoerythrobilin
[C-phycoerythrin alpha-subunit]-Cys84-phycoerythrobilin
show the reaction diagram
-
-
-
-
?
apo-[C-phycoerythrin beta-subunit] + (3E)-phycoerythrobilin
[C-phycoerythrin beta-subunit]-Cys84-phycoerythrobilin
show the reaction diagram
-
-
-
-
?
apo-[phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[phycocyanin beta-subunit]-Cys82-phycocyanobilin
show the reaction diagram
-
-
-
?
apo-[phycocyanin beta-subunit] + phycoerythrobilin
[phycocyanin beta-subunit]-Cys82-phycoerythrobilin
show the reaction diagram
-
-
-
?
apo-[phycocyanin beta-subunit] + phytochromobilin
[phycocyanin beta-subunit]-Cys82-phytochromobilin
show the reaction diagram
-
-
-
?
apo-[phycoerythrin alpha-subunit] + phycoerythrobilin
[phycoerythrin alpha-subunit]-Cys82-phycoerythrobilin
show the reaction diagram
apo-[phycoerythrin beta-subunit] + 3Z-phycoerythrobilin
[phycoerythrin beta-subunit]-Cys82-phycoerythrobilin
show the reaction diagram
apo-[phycoerythrin beta-subunit] + phycocyanobilin
[phycoerythrin beta-subunit]-Cys82-phycocyanobilin
show the reaction diagram
apo-[phycoerythrin beta-subunit] + phycoerythrobilin
[phycoerythrin beta-subunit]-Cys80-phycoerythrobilin
show the reaction diagram
-
CpeS bilin lyase efficiently ligates phycoerythrobilin to Cys80 of phycoerythrin beta-subunit
-
?
apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
apo allophycocyanin alpha-subunit + (2R,3E)-phycocyanobilin
[allophycocyanin alpha-subunit]-Cys81-phycocyanobilin
show the reaction diagram
apo allophycocyanin beta-subunit + (2R,3E)-phycocyanobilin
[allophycocyanin beta-subunit]-Cys81-phycocyanobilin
show the reaction diagram
apo C-phycocyanin beta-subunit + (2R,3E)-phycocyanobilin
[C-phycocyanin beta-subunit]-Cys82-phycocyanobilin
show the reaction diagram
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
show the reaction diagram
apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin
[phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NaCl
-
optimal concentration: 150 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Co2+
-
5 mM, complete inhibition
Cu2+
-
5 mM, complete inhibition
Fe2+
-
5 mM, 46% inhibition
imidazole
-
non-competitive
Mn2+
-
5 mM, 12% inhibition
Ni2+
-
5 mM, complete inhibition
Zn2+
-
5 mM, complete inhibition
additional information
-
no effect up to 20 mM EDTA
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00114 - 0.0027
(2R,3E)-phycocyanobilin
0.0000095
apo-[allophycocyanin alpha-subunit]
-
pH and temperature not specified in the publication, apo allophycocyanin alpha-subunit encoded by apcA1
-
0.000038
apo-[allophycocyanin beta-subunit]
-
pH and temperature not specified in the publication, apo allophycocyanin beta-subunit encoded by apcF
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000073 - 0.0000343
(2R,3E)-phycocyanobilin
0.0027
apo-[allophycocyanin alpha-subunit]
Anabaena sp.
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pH and temperature not specified in the publication, apo-[allophycocyanin alpha-subunit] encoded by apcA1
-
0.0024
apo-[allophycocyanin beta-subunit]
Anabaena sp.
-
pH and temperature not specified in the publication, apo-[allophycocyanin beta-subunit] encoded by apcF
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0029 - 0.0247
(2R,3E)-phycocyanobilin
196126
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
-
substrate: apo-[C-phycocyanin beta-subunit]
7.5 - 8
-
substrate: apo-[phycoerythrocyanin beta-subunit] mutant C155
7.5
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9.5
-
pH 6.5: about 50% of maximal activity, pH 9.5: about 75% of maximal activity, substrate: apo-[C-phycocyanin beta-subunit]
7 - 8.5
-
pH 7.0: about 80% of maximal activity, pH 7.0: about 80% of maximal activity, substrate: apo-[phycoerythrocyanin beta-subunit] mutant C155
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
40
-
substrate: apo-[phycoerythrocyanin beta-subunit] mutant C155
45
-
substrate: apo-[C-phycocyanin beta-subunit]
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 55
-
30C: about 55% of maximal activity, 55C: about 70% of maximal activity, substrate: apo-[C-phycocyanin beta-subunit]; 30C: about 75% of maximal activity, 55C: about 50% of maximal activity, substrate: apo-[phycoerythrocyanin beta-subunit] mutant C155
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
21900
2 * 21900, calculated
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to 2.8 A resolution. Enzyme is a 10-stranded beta barrel with two alpha helices and belongs to the lipocalin structural family
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli BL21 DE3
A8HTN2 and A8HTM2
expression in Escherichia coli; expression in Escherichia coli
overexpressed in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C51T
-
insoluble mutant enzyme
C63T
-
kcat/Km for (2R,3E)-phycocyanobilin is 1.2fold lower than wild-type value
G119C/W140V
-
insoluble mutant enzyme
H21T/H22V
-
kcat/Km for (2R,3E)-phycocyanobilin is 3fold lower than wild-type value
H22V
-
kcat/Km for (2R,3E)-phycocyanobilin is 2.5fold lower than wild-type value
H80L
-
kcat/Km for (2R,3E)-phycocyanobilin is 1.5fold lower than wild-type value
R147Q
-
kcat/Km for (2R,3E)-phycocyanobilin is 3fold lower than wild-type value
R169L
-
kcat/Km for (2R,3E)-phycocyanobilin is 1.7fold lower than wild-type value, loss of fidelity in chromophore attachment
R18V
-
kcat/Km for (2R,3E)-phycocyanobilin is 7.5fold lower than wild-type value
W14I
-
insoluble mutant enzyme
W69M
-
kcat/Km for (2R,3E)-phycocyanobilin is 1.1fold higher than wild-type value
W75S
-
kcat/Km for (2R,3E)-phycocyanobilin is 3fold lower than wild-type value
C51T
-
insoluble mutant enzyme
-
H21T/H22V
-
kcat/Km for (2R,3E)-phycocyanobilin is 3fold lower than wild-type value
-
H22V
-
kcat/Km for (2R,3E)-phycocyanobilin is 2.5fold lower than wild-type value
-
R18V
-
kcat/Km for (2R,3E)-phycocyanobilin is 7.5fold lower than wild-type value
-
W14I
-
insoluble mutant enzyme
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis