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Literature summary for 4.4.1.29 extracted from

  • Zhao, K.H.; Su, P.; Li, J.; Tu, J.M.; Zhou, M.; Bubenzer, C.; Scheer, H.
    Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120 (2006), J. Biol. Chem., 281, 8573-8581.
    View publication on PubMed
Search for more literature for 4.4.1.29

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Anabaena sp.

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 5 mM, complete inhibition Anabaena sp.
Cu2+ 5 mM, complete inhibition Anabaena sp.
Fe2+ 5 mM, 46% inhibition Anabaena sp.
Mn2+ 5 mM, 12% inhibition Anabaena sp.
additional information no effect up to 20 mM EDTA Anabaena sp.
Ni2+ 5 mM, complete inhibition Anabaena sp.
Zn2+ 5 mM, complete inhibition Anabaena sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, cosubstrate: apo-[phycoerythrocyanin beta-subunit] mutant C155I Anabaena sp.
0.0027
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, cosubstrate: apo-[C-phycocyanin beta-subunit] mutant C155I Anabaena sp.

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl optimal concentration: 150 mM Anabaena sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin Anabaena sp. the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin Anabaena sp. PCC 7120 the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin Anabaena sp. the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin Anabaena sp. PCC 7120 the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?

Organism

Organism UniProt Comment Textmining
Anabaena sp. Q8YZ70
-
-
Anabaena sp. PCC 7120 Q8YZ70
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Anabaena sp.

Reaction

Reaction Comment Organism Reaction ID
[allophycocyanin alpha-subunit]-Cys84-phycocyanobilin = apo-[allophycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin (3) Anabaena sp.
a
[allophycocyanin beta-subunit]-Cys84-phycocyanobilin = apo-[allophycocyanin beta-subunit] + (2R,3E)-phycocyanobilin (4) Anabaena sp.
a
[C-phycocyanin beta-subunit]-Cys84-phycocyanobilin = apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin (1) Anabaena sp.
a
[C-phycoerythrin alpha-subunit]-Cys84-phycoerythrobilin = apo-[C-phycoerythrin alpha-subunit] + (2R,3E)-phycoerythrobilin (5) Anabaena sp.
a
[C-phycoerythrin beta-subunit]-Cys84-phycoerythrobilin = apo-[C-phycoerythrin beta-subunit] + (2R,3E)-phycoerythrobilin (6) Anabaena sp.
a
[phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin = apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin (2) Anabaena sp.
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins Anabaena sp. [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin the enzyme attaches (2R,3E)-phycocyanobilin to native apo C-phycocyanin beta-subunit and to the C155I-mutant, but not to theC84S mutant Anabaena sp. [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins Anabaena sp. PCC 7120 [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin the enzyme attaches (2R,3E)-phycocyanobilin to native apo C-phycocyanin beta-subunit and to the C155I-mutant, but not to theC84S mutant Anabaena sp. PCC 7120 [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins Anabaena sp. [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin the enzyme attaches (2R,3E)-phycocyanobilin to native apo phycoerythrocyanin beta-subunit and to the C155I-mutant, but not to theC84S mutant Anabaena sp. [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins Anabaena sp. PCC 7120 [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin the enzyme attaches (2R,3E)-phycocyanobilin to native apo phycoerythrocyanin beta-subunit and to the C155I-mutant, but not to theC84S mutant Anabaena sp. PCC 7120 [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?

Synonyms

Synonyms Comment Organism
alr0617
-
 Anabaena sp.
cpeS
-
 Anabaena sp.
phycocyanobilin:Cys-beta84-phycobiliprotein lyase
-
 Anabaena sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 substrate: apo-[phycoerythrocyanin beta-subunit] mutant C155 Anabaena sp.
45
-
 substrate: apo-[C-phycocyanin beta-subunit] Anabaena sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 30°C: about 55% of maximal activity, 55°C: about 70% of maximal activity, substrate: apo-[C-phycocyanin beta-subunit] Anabaena sp.
30 55 30°C: about 75% of maximal activity, 55°C: about 50% of maximal activity, substrate: apo-[phycoerythrocyanin beta-subunit] mutant C155 Anabaena sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000011
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, cosubstrate: apo phycoerythrocyanin beta-subunit mutant C155I Anabaena sp.
0.000017
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, cosubstrate: apo C-phycocyanin beta-subunit mutant C155I Anabaena sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9 substrate: apo-[C-phycocyanin beta-subunit] Anabaena sp.
7.5 8 substrate: apo-[phycoerythrocyanin beta-subunit] mutant C155 Anabaena sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 pH 6.5: about 50% of maximal activity, pH 9.5: about 75% of maximal activity, substrate: apo-[C-phycocyanin beta-subunit] Anabaena sp.
7 8.5 pH 7.0: about 80% of maximal activity, pH 7.0: about 80% of maximal activity, substrate: apo-[phycoerythrocyanin beta-subunit] mutant C155 Anabaena sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0048
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, cosubstrate: apo-[phycoerythrocyanin beta-subunit] mutant C155I Anabaena sp.
0.0063
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, cosubstrate: apo-[C-phycocyanin beta-subunit] mutant C155I Anabaena sp.