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Literature summary for 4.4.1.29 extracted from

  • Kupka, M.; Zhang, J.; Fu, W.L.; Tu, J.M.; Bohm, S.; Su, P.; Chen, Y.; Zhou, M.; Scheer, H.; Zhao, K.H.
    Catalytic mechanism of S-type phycobiliprotein lyase: chaperone-like action and functional amino acid residues (2009), J. Biol. Chem., 284, 36405-36414.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.29

Protein Variants

Protein Variants Comment Organism
C51T insoluble mutant enzyme Anabaena sp.
C63T kcat/Km for (2R,3E)-phycocyanobilin is 1.2fold lower than wild-type value Anabaena sp.
G119C/W140V insoluble mutant enzyme Anabaena sp.
H21T/H22V kcat/Km for (2R,3E)-phycocyanobilin is 3fold lower than wild-type value Anabaena sp.
H22V kcat/Km for (2R,3E)-phycocyanobilin is 2.5fold lower than wild-type value Anabaena sp.
H80L kcat/Km for (2R,3E)-phycocyanobilin is 1.5fold lower than wild-type value Anabaena sp.
R147Q kcat/Km for (2R,3E)-phycocyanobilin is 3fold lower than wild-type value Anabaena sp.
R169L kcat/Km for (2R,3E)-phycocyanobilin is 1.7fold lower than wild-type value, loss of fidelity in chromophore attachment Anabaena sp.
R18V kcat/Km for (2R,3E)-phycocyanobilin is 7.5fold lower than wild-type value Anabaena sp.
W14I insoluble mutant enzyme Anabaena sp.
W69M kcat/Km for (2R,3E)-phycocyanobilin is 1.1fold higher than wild-type value Anabaena sp.
W75S kcat/Km for (2R,3E)-phycocyanobilin is 3fold lower than wild-type value Anabaena sp.

Inhibitors

Inhibitors Comment Organism Structure
imidazole non-competitive Anabaena sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00114
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, wild-type enzyme Anabaena sp.
0.00134
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme H21T/H22V Anabaena sp.
0.00138
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme H22V Anabaena sp.
0.00139
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme R169L Anabaena sp.
0.00139
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme W69M Anabaena sp.
0.00144
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme W75S Anabaena sp.
0.00155
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme C63T Anabaena sp.
0.0017
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme H80L Anabaena sp.
0.00229
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme R147Q Anabaena sp.
0.00254
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme R18V Anabaena sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin Anabaena sp.
-
 [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin Anabaena sp. PCC 7120
-
 [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?

Organism

Organism UniProt Comment Textmining
Anabaena sp. Q8YZ70
-
-
Anabaena sp. PCC 7120 Q8YZ70
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
-
 Anabaena sp. [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin
-
 Anabaena sp. PCC 7120 [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?

Synonyms

Synonyms Comment Organism
CpcS1
-
 Anabaena sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Anabaena sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0000073
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme R18V Anabaena sp.
0.0000095
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme H21T/H22V Anabaena sp.
0.0000106
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme W75S Anabaena sp.
0.0000119
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme H22V Anabaena sp.
0.0000169
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme R147Q Anabaena sp.
0.000018
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme R169L Anabaena sp.
0.0000246
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme H80L Anabaena sp.
0.000025
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, wild-type enzyme Anabaena sp.
0.000028
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme C63T Anabaena sp.
0.0000343
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme W69M Anabaena sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Anabaena sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0029
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme R18V Anabaena sp.
0.0071
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme H21T/H22V Anabaena sp.
0.0074
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme R147Q Anabaena sp.
0.0074
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme W75S Anabaena sp.
0.0086
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme H22V Anabaena sp.
0.0129
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme R169L Anabaena sp.
0.0147
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme H80L Anabaena sp.
0.018
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme C63T Anabaena sp.
0.0219
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, wild-type enzyme Anabaena sp.
0.0247
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, mutant enzyme W69M Anabaena sp.