4.3.99.3: 7-carboxy-7-deazaguanine synthase

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For detailed information about 7-carboxy-7-deazaguanine synthase, go to the full flat file.

7-carboxy-7-carbaguanine synthase, BsQueE, CDG synthase, queE, ToyC

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Results	in table
25241	AA Sequence
6	Cloned(Commentary)
13	Cofactor
3	Crystallization (Commentary)
10	General Information
2	KM Value [mM]
7	Metals/Ions
1	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
22	Organism
5	Pathway
18	PDB ID
3	pH Optimum
5	Purification (Commentary)
2	Reaction
14	Reference
24	Substrates and Products (Substrate)
2	Subunits
22	Synonyms
1	Systematic Name
1	Turnover Number [1/s]

Cloned on EC 4.3.99.3 - 7-carboxy-7-deazaguanine synthase

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expression in Escherichia coli

gene queE, expression of His-tagged QueE in Escherichia coli strain BL21(DE3) from plasmid pRM78

overexpression in Escherichia coli

recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)