Cloned (Comment) | Organism |
---|---|
gene queE, expression of His-tagged QueE in Escherichia coli strain BL21(DE3) from plasmid pRM78 | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-carboxy-5,6,7,8-tetrahydropterin | Bacillus subtilis | - |
7-carboxy-7-deazaguanine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
gene queE | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged QueE from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-carboxy-5,6,7,8-tetrahydropterin | - |
Bacillus subtilis | 7-carboxy-7-deazaguanine + NH3 | - |
? | |
6-carboxy-5,6,7,8-tetrahydropterin | substrate from a coupled reaction with Escherichia coli CPH4 synthase | Bacillus subtilis | 7-carboxy-7-deazaguanine + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
queE | - |
Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | dependent on. Members of the radical SAM superfamily contain a CX3CX2C-motif for binding a [4Fe-4S] cluster, which when reductively activated, cleaves SAM to 5?-deoxyadenosyl radical, which in turn initiates radical mediated transformations of the substrate | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
evolution | QueE is a member of the radical SAM organic radical generating enzyme superfamily. Members of the radical SAM superfamily contain a CX3CX2C-motif for binding a [4Fe-4S] cluster, which when reductively activated, cleaves SAM to 5'-deoxyadenosyl radical, which in turn initiates radical mediated transformations of the substrate | Bacillus subtilis |
metabolism | QueE is involved in the queuosine biosynthetic pathway of Bacillus subtilis | Bacillus subtilis |