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4.3.1.19: threonine ammonia-lyase

This is an abbreviated version!
For detailed information about threonine ammonia-lyase, go to the full flat file.

Word Map on EC 4.3.1.19

Reaction

2-aminobut-2-enoate
=
2-iminobutanoate

Synonyms

BsBTD1, CgBTD1, CgCTD, EC 4.2.1.16, EcBTD2, EcCTD, FgIlv1, GSU0486, ilvA, L-TD, L-TDH, L-threonine deaminase, L-threonine dehydratase, MSMEG3183, OMR1, PpBTD2, pTD2, SaBTD1, SaCTD, SgBTD1, SlTD1, SlTD2, sp0454, TD, TD1, TD2, tdcB, TDH, TH, Thr ammonia-lyase, threonine ammonia-lyase, Threonine deaminase, threonine deaminase/dehydratase, threonine dehydrase, threonine dehydratase, threonine dehydratase/deaminase

ECTree

     4 Lyases
         4.3 Carbon-nitrogen lyases
             4.3.1 Ammonia-lyases
                4.3.1.19 threonine ammonia-lyase

Molecular Weight

Molecular Weight on EC 4.3.1.19 - threonine ammonia-lyase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
-
determined by gel-filtration in the presence of CMP
100000 - 115000
-
gel filtration
106000
-
gel filtration
118000
-
gel filtration
120000
140000
-
native PAGE, in the presence of the allosteric effector isoleucine
147000
-
sedimentation equilibrium ultracentrifugation
160000
-
gel filtration
180000
gel filtration
190000
200000
201000
-
equilibrium sedimentation
203800
-
sedimentation equilibrium experiments
210000
214000
-
meniscus depletion equilibrium sedimentation, analytical ultracentrifugation
228000
-
calculation from sedimentation and diffusion data
230000
-
gel filtration
250000
-
dehydratase II, gel filtration
268000
-
native PAGE, in the absence of allosteric effector
30000
-
4 * 30000, SDS-PAGE
32000
36000
370000
-
Ile-sensitive enzyme form, gel filtration
38000
-
x * 38000, SDS-PAGE
39000
-
4 * 39000, SDS-PAGE
40000
molecular mass of processed TD2 determined by SDS-PAGE
45000
46000
-
4 * 46000, SDS-PAGE
46599
-
4 * 46599, calculation from nucleotide sequence
49000
-
4 * 49000, SDS-PAGE
49800
-
4 * 49800, equilibrium sedimentation of the enzyme dialyzed against 6 M guanidine hydrochloride
50000
-
4 * 50000, SDS-PAGE
53000
-
4 * 53000, the enzyme exists in two distinct catalytically active species: a tetramer sensitive to L-Ile inhibition and a dimer insensitive to L-Ile inhibition, SDS-PAGE
55000
56000
-
4 * 56000, SDS-PAGE
57000
-
4 * 57000, SDS-PAGE
58000
-
4 * 58000, SDS-PAGE
59567
59800
-
4 * 59800, SDS-PAGE
66180
predicted molecular mass of SlTD1