4.3.1.19: threonine ammonia-lyase
This is an abbreviated version!
For detailed information about threonine ammonia-lyase, go to the full flat file.
Word Map on EC 4.3.1.19
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4.3.1.19
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valine
-
pyridoxal
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l-isoleucine
-
4.2.1.16
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alpha-ketobutyrate
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2-ketobutyrate
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l-serine
-
homoserine
-
isoleucine-valine
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acetohydroxy
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acetolactate
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citramalate
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attenuata
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feedback-resistant
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2-oxobutyrate
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acetohydroxyacid
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sinoatrial
-
molecular biology
-
synthesis
- 4.3.1.19
- valine
- pyridoxal
- l-isoleucine
-
4.2.1.16
- alpha-ketobutyrate
- 2-ketobutyrate
- l-serine
- homoserine
-
isoleucine-valine
-
acetohydroxy
- acetolactate
- citramalate
- attenuata
-
feedback-resistant
- 2-oxobutyrate
-
acetohydroxyacid
-
sinoatrial
- molecular biology
- synthesis
Reaction
Synonyms
BsBTD1, CgBTD1, CgCTD, EC 4.2.1.16, EcBTD2, EcCTD, FgIlv1, GSU0486, ilvA, L-TD, L-TDH, L-threonine deaminase, L-threonine dehydratase, MSMEG3183, OMR1, PpBTD2, pTD2, SaBTD1, SaCTD, SgBTD1, SlTD1, SlTD2, sp0454, TD, TD1, TD2, tdcB, TDH, TH, Thr ammonia-lyase, threonine ammonia-lyase, Threonine deaminase, threonine deaminase/dehydratase, threonine dehydrase, threonine dehydratase, threonine dehydratase/deaminase
ECTree
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Molecular Weight
Molecular Weight on EC 4.3.1.19 - threonine ammonia-lyase
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100000
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determined by gel-filtration in the presence of CMP
120000
140000
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native PAGE, in the presence of the allosteric effector isoleucine
190000
200000
210000
214000
-
meniscus depletion equilibrium sedimentation, analytical ultracentrifugation
32000
36000
45000
49800
-
4 * 49800, equilibrium sedimentation of the enzyme dialyzed against 6 M guanidine hydrochloride
53000
-
4 * 53000, the enzyme exists in two distinct catalytically active species: a tetramer sensitive to L-Ile inhibition and a dimer insensitive to L-Ile inhibition, SDS-PAGE
55000
59567
120000
-
determined by gel-filtration in the presence of AMP
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determined by gel-filtration in the absence of CMP and AMP
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2 * 59567, mass spectrometry, addition of isoleucine induces dimerization, tetrmerization is restored by addition of high valine concentration