Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-Val | activates | Candida maltosa | |
phosphate | increases activity | Candida maltosa | |
phosphate | maximal stimulation at 200 mM | Candida maltosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | - |
Candida maltosa | |
Ile | 50% inhibition at 0.14 mM Ile, 98% inhibition at 1 mM Ile | Candida maltosa | |
PCMB | - |
Candida maltosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
L-Thr | - |
Candida maltosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for divalent cations | Candida maltosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
4 * 46000, SDS-PAGE | Candida maltosa |
190000 | - |
gel filtration | Candida maltosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida maltosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Candida maltosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Candida maltosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-homoserine | - |
Candida maltosa | 2-oxobutanoate + NH3 + H2O | - |
? | |
L-serine | - |
Candida maltosa | pyruvate + NH3 | - |
? | |
L-threonine | - |
Candida maltosa | 2-oxobutanoate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 46000, SDS-PAGE | Candida maltosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
- |
Candida maltosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.1 | 9.6 | 50% of maximal activity at pH 8.1 and 9.6 | Candida maltosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | cofactor | Candida maltosa | |
pyridoxal 5'-phosphate | Km: 0.00028 mM | Candida maltosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
Ile | - |
Candida maltosa |