Any feedback?
Literature summary for 4.3.1.19 extracted from
- Crystal structures of Salmonella typhimurium biodegradative threonine deaminase and its complex with CMP provide structural insights into ligand-induced oligomerization and enzyme activation (2006), J. Biol. Chem., 281, 39630-39641.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| AMP | - |
Salmonella enterica subsp. enterica serovar Typhimurium |  |
| CMP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the pRSETC vector for expression in Escherichia coli BL21DE3 pLysS cells | Salmonella enterica subsp. enterica serovar Typhimurium |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| data for two crystal forms are collected to resolutions of 2.2 and 1.7 A, crystals obtainted in the presence of CMP diffract to a resolution of 3-3.5 A, in the presence of AMP poorly | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16 | - |
L-threonine | in the presence of 5 mM AMP | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 32 | - |
L-threonine | in the presence of 5 mM CMP | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 123 | - |
L-threonine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
determined by gel-filtration in the absence of CMP and AMP | Salmonella enterica subsp. enterica serovar Typhimurium |
| 100000 | - |
determined by gel-filtration in the presence of CMP | Salmonella enterica subsp. enterica serovar Typhimurium |
| 120000 | - |
determined by gel-filtration in the presence of AMP | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-threonine | Salmonella enterica subsp. enterica serovar Typhimurium | - |
2-oxobutanoate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a nickel-nitrilotriacetic acid affinity column | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-threonine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 2-oxobutanoate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | native structure | Salmonella enterica subsp. enterica serovar Typhimurium |
| tetramer | in the complex with CMP | Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Threonine deaminase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
activity assay | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
activity assay | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
