Cloned (Comment) | Organism |
---|---|
a modified SlTD2 cDNA is cloned into the pET30a+ vector producing a truncated form of SlTD2 lacking the 51 amino acids corresponding to the N-terminal chloroplast-targeting sequence | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
L-threonine | processed TD2 | Solanum lycopersicum | |
3 | - |
L-serine | processed TD2 | Solanum lycopersicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
molecular mass of processed TD2 determined by SDS-PAGE | Solanum lycopersicum |
55000 | - |
determined by Western blot analysis, mature TD2 | Solanum lycopersicum |
66180 | - |
predicted molecular mass of SlTD1 | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine | Solanum lycopersicum | - |
2-oxobutanoate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | P25306 | - |
- |
Purification (Comment) | Organism |
---|---|
using a Sephadex G-25, a DEAE-cellulose DE52, a HiLoad26/60 Superdex 200 and a HiPrep 16/10 column | Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | - |
Solanum lycopersicum | pyruvate + NH3 | - |
? | |
L-threonine | - |
Solanum lycopersicum | 2-oxobutanoate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pTD2 | processed form of TD2, the C-terminal regulatory domain is removed | Solanum lycopersicum |
SlTD1 | isoform, involved in isoleucine biosynthesis in plants | Solanum lycopersicum |
SlTD2 | isoform, for plant defense the protein is involved in threonine degradation in leaf eating insects | Solanum lycopersicum |
TD | - |
Solanum lycopersicum |
TD1 | - |
Solanum lycopersicum |
TD2 | - |
Solanum lycopersicum |
Threonine deaminase | - |
Solanum lycopersicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
58 | - |
- |
Solanum lycopersicum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
active over a wide range of temperatures, optimal enzyme activity is observed at 58°C | Solanum lycopersicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme is highly active in an alkaline pH range, little or no activity is observed at pH values below 6.0 | Solanum lycopersicum |