4.3.1.16: threo-3-hydroxy-L-aspartate ammonia-lyase

This is an abbreviated version!
For detailed information about threo-3-hydroxy-L-aspartate ammonia-lyase, go to the full flat file.

Reaction

Synonyms

D-THA DH, D-threo-3-hydroxyaspartate dehydratase, L-THA DH, L-threo-3-hydroxyaspartate dehydratase, threo-3-hydroxyaspartate ammonia-lyase, threo-3-hydroxyaspartate dehydratase

ECTree

     4 Lyases

         4.3 Carbon-nitrogen lyases

             4.3.1 Ammonia-lyases

                4.3.1.16 threo-3-hydroxy-L-aspartate ammonia-lyase

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Results	in table
2	Activating Compound
1107	AA Sequence
1	Application
1	CAS Registry Number
3	Cloned(Commentary)
2	Cofactor
1	Crystallization (Commentary)
13	Inhibitors
2	kcat/KM [mM/s]
3	Ki Value [mM]
5	KM Value [mM]
9	Metals/Ions
3	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
4	Organism
2	pH Optimum
1	Protein Variants
4	Purification (Commentary)
1	Reaction
2	Reaction Type
4	Reference
5	Specific Activity [micromol/min/mg]
6	Substrates and Products (Substrate)
2	Subunits
7	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
2	Temperature Stability [°C]
2	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 4.3.1.16 - threo-3-hydroxy-L-aspartate ammonia-lyase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

erythro-3-hydroxy-D-aspartate

oxaloacetate + NH3

Pseudomonas sp. N99

L0N5C4

-

747320

-

-

?

L-threo-3-hydroxyaspartate

oxaloacetate + NH3

2 entries

threo-3-hydroxy-L-aspartate

oxaloacetate + NH3

Pseudomonas sp. N99

L0N5C4

-

747320

-

-

?

additional information

?

-

2 entries

L-threo-3-hydroxyaspartate

oxaloacetate + NH3

Pseudomonas sp.

-

no substrates are D-threo or D,L-erythro-3-hydroxyaspartate

285204

-

285204

?

L-threo-3-hydroxyaspartate

oxaloacetate + NH3

Pseudomonas sp. T62

A4F2N8

-

692924

-

-

r

additional information

?

-

Pseudomonas sp. N99

L0N5C4

no activity with erythro-3-hydroxy-L-aspartate and threo-3-hydroxy-D-aspartate

747320

-

-

?

additional information

?

-

Pseudomonas sp. T62

A4F2N8

none of the other 3-hydroxy amino acids tested (DL-erythro- and D-threo-3-hydroxyaspartate, D-threonine, L-threonine, DL-allo-threonine, D-serine, L-serine, and DL-phenylserine) is a substrate for this enzyme, either at 5 mM or 50 mM, as in the case of the enzyme from the original strain

692924

-

-

?