4.3.1.16: threo-3-hydroxy-L-aspartate ammonia-lyase

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For detailed information about threo-3-hydroxy-L-aspartate ammonia-lyase, go to the full flat file.

Word Map on EC 4.3.1.16

4.3.1.16

l-erythro-3-hydroxyaspartate

pyridoxal

racemase

protein-1

threonine

delftia

micromol

fold-type

soil-isolated

chicken

5'-phosphate

pombe

hydroxylamine

dehydration

5'-phosphate-dependent

d-serine

edta

schizosaccharomyces

synthesis

Reaction

Synonyms

D-THA DH, D-threo-3-hydroxyaspartate dehydratase, L-THA DH, L-threo-3-hydroxyaspartate dehydratase, threo-3-hydroxyaspartate ammonia-lyase, threo-3-hydroxyaspartate dehydratase

ECTree

4 Lyases

4.3 Carbon-nitrogen lyases

4.3.1 Ammonia-lyases

4.3.1.16 threo-3-hydroxy-L-aspartate ammonia-lyase

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Results	in table
2	Activating Compound
1107	AA Sequence
1	Application
1	CAS Registry Number
3	Cloned(Commentary)
2	Cofactor
1	Crystallization (Commentary)
13	Inhibitors
2	kcat/KM [mM/s]
3	Ki Value [mM]
5	KM Value [mM]
9	Metals/Ions
3	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
4	Organism
2	pH Optimum
1	Protein Variants
4	Purification (Commentary)
1	Reaction
2	Reaction Type
4	Reference
5	Specific Activity [micromol/min/mg]
6	Substrates and Products (Substrate)
2	Subunits
7	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
2	Temperature Stability [°C]
2	Turnover Number [1/s]

Purification

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Purification on EC 4.3.1.16 - threo-3-hydroxy-L-aspartate ammonia-lyase

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2 entries

after centrifugation of cultivated cell culture with the recombinant enzyme, resulting supernatant is applied to a Ni-sepharose column connected to a fast protein liquid chromatography system. The column is equilibrated with buffer supplemented with 20 mM imidazole. The enzyme is eluted with imidazole gradient.

Pseudomonas sp. T62

A4F2N8

692924

recombinant His-tagged enzyme

Pseudomonas sp. N99

L0N5C4

747320