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4.2.3.82: alpha-santalene synthase

This is an abbreviated version!
For detailed information about alpha-santalene synthase, go to the full flat file.

Word Map on EC 4.2.3.82

Reaction

(2E,6E)-farnesyl diphosphate
=
(+)-alpha-santalene
+
diphosphate

Synonyms

SanSyn, santalene synthase, SaSSy, SspiSy, SSy, SuSSy, Tps2-1

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.82 alpha-santalene synthase

Purification

Purification on EC 4.2.3.82 - alpha-santalene synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by streptomycin sulfate and ammonium sulfate fractionation, desalting gel filtration, and anion exchange chromatography, followed by another step of ammonium sulfate fractionation and desalting gel filtration to near homogeneity
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) ickel affinity chromatography, dialysis, and ultrafiltration to near homogeneity
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) ickel affinity chromatography, dialysis, anion exchange chromatography, and gel filtration to near homogeneity