Information on EC 4.2.3.82 - alpha-santalene synthase

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The expected taxonomic range for this enzyme is: Gunneridae

EC NUMBER
COMMENTARY hide
4.2.3.82
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RECOMMENDED NAME
GeneOntology No.
alpha-santalene synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate = (+)-alpha-santalene + diphosphate
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
santalene biosynthesis II
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl diphosphate lyase (cyclizing, (+)-alpha-santalene-forming)
The enzyme synthesizes a mixture of sesquiterpenoids from (2E,6E)-farnesyl diphosphate. As well as (+)-alpha-santalene, (-)-beta-santalene and (-)-exo-alpha-bergamotene are formed with traces of (+)-epi-beta-santalene. See EC 4.2.3.83 [(-)-beta-santalene synthase], and EC 4.2.3.81 [(-)-exo-alpha-bergamotene synthase]. cf. EC 4.2.3.50 alpha-santalene synthase [(2Z,6Z)-farnesyl diphosphate cyclizing]
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(+)-alpha-santalene + diphosphate
show the reaction diagram
(2E,6E)-farnesyl diphosphate
alpha-santalene + diphosphate
show the reaction diagram
2 (2E,6E)-farnesyl diphosphate
(+)-alpha-santalene + trans-alpha-bergamotene + 2 diphosphate
show the reaction diagram
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amounts of the sesquiterpenes alpha-santalene and trans-alpha-bergamotene produced are,respectively,1.45 and0.177 mg/l
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(+)-alpha-santalene + diphosphate
show the reaction diagram
(2E,6E)-farnesyl diphosphate
alpha-santalene + diphosphate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0014
(2E,6E)-farnesyl diphosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.34 - 2.6
(2E,6E)-farnesyl diphosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
242.9 - 1857
(2E,6E)-farnesyl diphosphate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6
calculated
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
verly low expression
Manually annotated by BRENDA team
expression in mature outer and transition zone is higher by 10% as compared to immature wood sample
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli; heterologous expression of a His6-tagged protein in Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
construction of a Saccharomyces cerevisiae strain capable of producing high levels of alpha-santalene through a rationally designed metabolic engineering approach.Optimal sesquiterpene production is obtained by modulating the expression of one of the keymetabolic steps of the mevalonate pathway, squalene synthase Erg9. Further optimization yields a final titer of 92 mg/l of alpha-santalene