Information on EC 4.2.3.82 - alpha-santalene synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Santalum

EC NUMBER
COMMENTARY
4.2.3.82
-
RECOMMENDED NAME
GeneOntology No.
alpha-santalene synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(2E,6E)-farnesyl diphosphate = (+)-alpha-santalene + diphosphate
show the reaction diagram
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
santalene biosynthesis II
-
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl diphosphate lyase (cyclizing, (+)-alpha-santalene-forming)
The enzyme synthesizes a mixture of sesquiterpenoids from (2E,6E)-farnesyl diphosphate. As well as (+)-alpha-santalene, (-)-beta-santalene and (-)-exo-alpha-bergamotene are formed with traces of (+)-epi-beta-santalene. See EC 4.2.3.83 [(-)-beta-santalene synthase], and EC 4.2.3.81 [(-)-exo-alpha-bergamotene synthase]. cf. EC 4.2.3.50 alpha-santalene synthase [(2Z,6Z)-farnesyl diphosphate cyclizing]
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SaSSy
E3W202
-
SspiSy
E3W204
-
SSy
E3W202
-
SSy
E3W204
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(+)-alpha-santalene + diphosphate
show the reaction diagram
E3W202
-
-
-
?
(2E,6E)-farnesyl diphosphate
(+)-alpha-santalene + diphosphate
show the reaction diagram
E3W203
-
-
-
?
(2E,6E)-farnesyl diphosphate
(+)-alpha-santalene + diphosphate
show the reaction diagram
E3W204
-
-
-
?
(2E,6E)-farnesyl diphosphate
(+)-alpha-santalene + diphosphate
show the reaction diagram
E3W202
when incubated in the presence of 10 mM Mg2+ the enzyme converts (2E,6E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
-
-
?
(2E,6E)-farnesyl diphosphate
(+)-alpha-santalene + diphosphate
show the reaction diagram
E3W203
when incubated in the presence of 10 mM Mg2+ the enzyme converts (2E,6E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
-
-
?
(2E,6E)-farnesyl diphosphate
(+)-alpha-santalene + diphosphate
show the reaction diagram
E3W204
when incubated in the presence of 10 mM Mg2+ the enzyme converts (2E,6E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
-
-
?
(2E,6E)-farnesyl diphosphate
(+)-alpha-santalene + diphosphate
show the reaction diagram
E3W202
when incubated in the presence of 10 mM Mg2+ the enzyme converts (2Z,6Z)-farnesyl diphosphate into alpha-endo-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene and beta-santalene
-
-
?
(2E,6E)-farnesyl diphosphate
alpha-santalene + diphosphate
show the reaction diagram
E3W203
-
products are alpha-santalene, beta-, and epi-beta-santalene, alpha-exo-bergamotene, as well as traces of alpha- and beta-farnesene
-
?
(2E,6E)-farnesyl diphosphate
alpha-santalene + diphosphate
show the reaction diagram
E3W204
-
products are alpha-santalene, beta-, and epi-beta-santalene, alpha-exo-bergamotene, as well as traces of alpha- and beta-farnesene
-
?
(2E,6E)-farnesyl diphosphate
alpha-santalene + diphosphate
show the reaction diagram
E3W202
-
products are alpha-santalene, beta-, and epi-beta-santalene, exo-alpha-bergamotene, as well as traces of alpha- and beta-farnesene
-
?
additional information
?
-
E3W202
Santalum album Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of alpha-endo-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene. The product profile resulting from incubations of SaSSy with (E,E)-FPP resembles the authentic essential oil of Santalum album more closely than that of the (Z,Z)-FPP incubations
-
-
-
additional information
?
-
E3W203
Santalum austrocaledonicum Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of endo-alpha-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene
-
-
-
additional information
?
-
E3W204
Santalum spicatum Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of endo-alpha-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene
-
-
-
additional information
?
-
E3W202
Santalum album Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of alpha-endo-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(+)-alpha-santalene + diphosphate
show the reaction diagram
E3W202
-
-
-
?
(2E,6E)-farnesyl diphosphate
(+)-alpha-santalene + diphosphate
show the reaction diagram
E3W203
-
-
-
?
(2E,6E)-farnesyl diphosphate
(+)-alpha-santalene + diphosphate
show the reaction diagram
E3W204
-
-
-
?
(2E,6E)-farnesyl diphosphate
alpha-santalene + diphosphate
show the reaction diagram
E3W203
-
products are alpha-santalene, beta-, and epi-beta-santalene, alpha-exo-bergamotene, as well as traces of alpha- and beta-farnesene
-
?
(2E,6E)-farnesyl diphosphate
alpha-santalene + diphosphate
show the reaction diagram
E3W204
-
products are alpha-santalene, beta-, and epi-beta-santalene, alpha-exo-bergamotene, as well as traces of alpha- and beta-farnesene
-
?
(2E,6E)-farnesyl diphosphate
alpha-santalene + diphosphate
show the reaction diagram
E3W202
-
products are alpha-santalene, beta-, and epi-beta-santalene, exo-alpha-bergamotene, as well as traces of alpha- and beta-farnesene
-
?
additional information
?
-
E3W202
Santalum album Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of alpha-endo-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene. The product profile resulting from incubations of SaSSy with (E,E)-FPP resembles the authentic essential oil of Santalum album more closely than that of the (Z,Z)-FPP incubations
-
-
-
additional information
?
-
E3W203
Santalum austrocaledonicum Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of endo-alpha-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene
-
-
-
additional information
?
-
E3W204
Santalum spicatum Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of endo-alpha-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
E3W202
required; when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
Mg2+
E3W203
required; when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
Mg2+
E3W204
required; when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
Mn2+
E3W202
when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
Mn2+
E3W203
incubation in presence of Mn2+ yields mainly alpha-exo-bergamotene; when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
Mn2+
E3W204
incubation in presence of Mn2+ yields mainly exo-alpha-bergamotene; when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0014
-
(2E,6E)-farnesyl diphosphate
E3W202
30C, pH 8.0; pH 7.4, 25C
0.0014
-
(2E,6E)-farnesyl diphosphate
E3W203
30C, pH 8.0; pH 7.4, 25C
0.0014
-
(2E,6E)-farnesyl diphosphate
E3W204
30C, pH 8.0; pH 7.4, 25C
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.34
-
(2E,6E)-farnesyl diphosphate
E3W202
30C, pH 8.0; pH 7.4, 25C
0.91
-
(2E,6E)-farnesyl diphosphate
E3W203
30C, pH 8.0; pH 7.4, 25C
2.6
-
(2E,6E)-farnesyl diphosphate
E3W204
30C, pH 8.0; pH 7.4, 25C
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
242.9
-
(2E,6E)-farnesyl diphosphate
E3W202
pH 7.4, 25C
263972
650
-
(2E,6E)-farnesyl diphosphate
E3W203
pH 7.4, 25C
263972
1857
-
(2E,6E)-farnesyl diphosphate
E3W204
pH 7.4, 25C
263972
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.4
-
E3W202
assay at
7.4
-
E3W203
assay at
7.4
-
E3W204
assay at
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
E3W202
assay at
25
-
E3W203
assay at
25
-
E3W204
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
66000
-
E3W202
-
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
E3W202
x * 65163, calculated from sequence; x * 66000, SDS-PAGE of recombinant His-tagged protein
?
E3W203
x * 65268, calculated from sequence
?
E3W204
x * 65407, calculated from sequence
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli; heterologous expression of a His6-tagged protein in Escherichia coli
E3W202
expression in Escherichia coli; heterologous expression of a His6-tagged protein in Escherichia coli
E3W203
expression in Escherichia coli; heterologous expression of a His6-tagged protein in Escherichia coli
E3W204