4.2.3.46: alpha-farnesene synthase
This is an abbreviated version!
For detailed information about alpha-farnesene synthase, go to the full flat file.
Reaction
Synonyms
(E,E)-alpha-farnesene synthase, AdAFS1, AFS, AFS-1, AFS1, alpha farnesene synthase 1, alpha-farnesene synthase, alpha-farnesene synthase-1, E,E-alpha-farnesene synthase, MdAFS1, sesquiterpene synthase, TPS02, TPS03, TPS2, Tps7
ECTree
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Engineering
Engineering on EC 4.2.3.46 - alpha-farnesene synthase
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D326A
alpha-farnesene synthase, monoterpene synthase and prenyltransferase activities are lost in the mutant
D326A/D330A
alpha-farnesene synthase, monoterpene synthase and prenyltransferase activities are lost in the mutant
D484A
S485A
S487A
S487K
S488A
85% loss of sesquiterpene synthase activity compared with wild-type enzyme when K+ is present
D484A
85% loss of sesquiterpene synthase activity compared with wild-type enzyme. Little change in K+-independent activity
exhibits marginally increased sesquiterpene synthase activities, and an approximate 2fold increase in monoterpene synthase activity compared with the wild-type enzyme
S485A
mutant exhibits marginally increased sesquiterpene synthase activities, and an approximate 2fold increase in monoterpene synthase activity compared with the wild-type enzyme
95% decrease in sesquiterpene synthase activity compared with wild-type enzyme when K+ is present
S487A
95% decrease in sesquiterpene synthase activity compared with wild-type enzyme. Little change in K+-independent activity
The S487K mutant has 35-45% of the wild-type activity with farnesyl diphosphate, with no significant alterations in the alpha-farnesene isomer ratios produced and a small decrease in catalytic efficiency
S487K
mutant has 3545% of the wild-type activity with farnesyl diphosphate, with no significant alterations in the alpha-farnesene isomer ratios produced and a small decrease in catalytic efficiency
mutant shows decreases in both sesqui- and monoterpene synthase activities compared with the WT enzyme, with mono-TPS activity being reduced more than sesquiterpene synthase activity. Sesquiterpene synthase (alpha-farnesene) products produced by the mutated and wild-type enzymes are identical, there are no significant alterations in the ratios of the alpha-farnesene isomers produced.
S488A
mutant shows decreases in both sesqui- and mono-terpene synthases activities, compared with the wild-type enzyme, with mono-terpene synthases activity being reduced more than sesqui-terpene synthases activity