4.2.3.46: alpha-farnesene synthase

This is an abbreviated version!
For detailed information about alpha-farnesene synthase, go to the full flat file.

Word Map on EC 4.2.3.46

4.2.3.46

apple

terpene

sesquiterpene

synthases

monoterpene

domestica

herbivores

conifer

e-beta-ocimene

malus

linalool

terpenoids

synthesis

agriculture

Reaction

Synonyms

(E,E)-alpha-farnesene synthase, AdAFS1, AFS, AFS-1, AFS1, alpha farnesene synthase 1, alpha-farnesene synthase, alpha-farnesene synthase-1, E,E-alpha-farnesene synthase, MdAFS1, sesquiterpene synthase, TPS02, TPS03, TPS2, Tps7

ECTree

4 Lyases

4.2 Carbon-oxygen lyases

4.2.3 Acting on phosphates

4.2.3.46 alpha-farnesene synthase

Advanced search results

Do not include text mining results

Include

results (more...)

Include

results (more...)

Results	in table
35	AA Sequence
2	Application
9	Cloned(Commentary)
6	Expression
2	General Information
3	Inhibitors
4	kcat/KM [mM/s]
5	KM Value [mM]
6	Localization
13	Metals/Ions
3	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
16	Organism
3	Pathway
4	pH Optimum
1	pH Range
1	pH Stability
1	pI Value
12	Protein Variants
3	Purification (Commentary)
1	Reaction
15	Reference
1	Renatured (Commentary)
15	Source Tissue
2	Specific Activity [micromol/min/mg]
26	Substrates and Products (Substrate)
2	Subunits
20	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Stability [°C]
26	Turnover Number [1/s]

Cloned

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Cloned on EC 4.2.3.46 - alpha-farnesene synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide

Go to Cloned (commentary) Search

Malus domestica

Q84LB2

698061

After screening a cDNA library generated from the peel tissue mRNA, a full-length terpene synthase cDNA 1931 nucleotides long is isolated (hot phenol RNA extraction protocol is used). The 1728-bp open reading frame encodes the 576 amino acid protein. Expression of the apple gene in Escherichia coli.

Malus domestica

Q84LB2

694765

expressed in Escherichia coli

Malus domestica

Q84LB2

700662

expressed in Escherichia coli BL21 (DE3) CodonPlus cells and transgenic hairy roots of Glycine max

Glycine max

748904

expression in Escherichia coli

2 entries

exprsssion in Escherichia coli and Saccharomyces cerevisiae

Ricinus communis

B9RXW0

720640

heterologously expressed in Escherichia coli

Actinidia deliciosa

C7SHN9

699267

mutated enzymes are generated using the QuickChange II site-directed mutagenesis kit. The PCR-based mutagenesis protocol is performed using pET-30a harbouring the MdAFS1 cDNAs as template. The single-site mutant enzymes overexpressed in Escherichia coli.

Malus domestica

B2ZZ11, Q84LB2

693257