4.2.3.29: ent-sandaracopimaradiene synthase
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For detailed information about ent-sandaracopimaradiene synthase, go to the full flat file.
Reaction
Synonyms
ent-(sandaraco)pimar-8(14),15-diene synthase, ent-kaurene synthase like 10, ent-sandaracopimaradiene synthase, KS like 10, KS(-like) enzyme, KS(L), KSL10, KSL10i, KSL10j, OrKSL10i, OrKSL10j, OsKS10, OsKSL10, RcKS(L), RcKSL3
ECTree
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General Information
General Information on EC 4.2.3.29 - ent-sandaracopimaradiene synthase
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evolution
metabolism
LC-MS/MS analyses show that oryzalexins A and C are not detected in either accession W0106 or W1943. Oryzalexins E and F, which are not detected in the two Or accessions, are also undetectable in Oryza sativa cv. Nipponbare. OrKSL10 and OrKSL10ind cannot usually produce ent-sandaracopimaradiene strongly suggesting that no accumulation of oryzalexins A-F is possible in either of the two Or accessions
physiological function
additional information
enzymes KSLs belong to the type A/class I terpene synthase family
evolution
enzymes KSLs belong to the type A/class I terpene synthase family
evolution
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plants have extensively diversified their arsenal of labdane-related diterpenoids (LRDs), in part via gene duplication and neo-functionalization of the ancestral ent-kaurene synthase (KS) required for gibberellin metabolism. Ricinus communis contains 4 RcKSL isozymes, molecular phylogenetic analysis indicates that RcKS(L)1 is significantly more closely related to dicotyl ent-kaurene synthases (KSs), while the other three, RcKSL2-4, cluster separately. RcKSL2-4 are in close proximity to each other, within a region of 65 kb, with RcKSL2 and 4 occurring as a tandem gene pair. RcKS(L)1 is the only isozyme to react with ent-CPP producing small amounts of ent-kaurene, and is referred to as RcKS1. Both RcKSL2 and RcKSL3 also are selectively reacting with ent-CPP with RcKSL2 producing primarily ent-trachylobane (70%) as well as smaller amounts of ent-kaurene (30%), and RcKSL3 producing ent-sandaracopimaradiene (94%) (EC 4.2.3.29) along with small amounts of ent-labdatriene (3%) and ent-pimaradiene (3%). RcKSL4 seems to be inactive, with no products evident from any substrate, but upon synthesis of a codon-optimized gene for the corrected aa sequence and functional analysis, RcKSL4 selectively reacts with ent-CPP and produces largely ent-beyerene (95%) along with very small amounts of ent-atiserene (4%) and ent-kaurene (1%). The enzymes belong to the terpene synthase family and is a class I diterpene synthase
OrKSL10j and OrKSL10ind encode ent-sandaracopimaradiene synthase responsible for the biosynthesis of oryzalexins A-F
physiological function
OsKSL10 encodes ent-sandaracopimaradiene synthase responsible for the biosynthesis of oryzalexins A-F, but oryzalexins E and F are undetectable in Oryza sativa cv. Nipponbare, they are also undetectable in Oryza rufipogon accessions W106 and W1943
recombinant isozymes OrKSL10j (from cv. W106) and OrKSL10ind (from cv. W1943) have similar activity
additional information
recombinant isozymes OrKSL10j (from cv. W106) and OrKSL10ind (from cv. W1943) have similar activity
additional information
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recombinant isozymes OrKSL10j (from cv. W106) and OrKSL10ind (from cv. W1943) have similar activity