Information on EC 4.2.3.29 - ent-sandaracopimaradiene synthase

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The expected taxonomic range for this enzyme is: Oryza sativa

EC NUMBER
COMMENTARY hide
4.2.3.29
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RECOMMENDED NAME
GeneOntology No.
ent-sandaracopimaradiene synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ent-copalyl diphosphate = ent-sandaracopimara-8(14),15-diene + diphosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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diterpene phytoalexins precursors biosynthesis
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Diterpenoid biosynthesis
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diterpene phytoalexins precursors biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
ent-copalyl-diphosphate diphosphate-lyase [ent-sandaracopimara-8(14),15-diene-forming]
ent-Sandaracopimaradiene is a precursor of the rice oryzalexins A-F. Phytoalexins are diterpenoid secondary metabolites that are involved in the defense mechanism of the plant, and are produced in response to pathogen attack through the perception of elicitor signal molecules such as chitin oligosaccharide, or after exposure to UV irradiation. As a minor product, this enzyme also forms ent-pimara-8(14),15-diene, which is the sole product of EC 4.2.3.30, ent-pimara-8(14),15-diene synthase. ent-Pimara-8(14),15-diene is not a precursor in the biosynthesis of either gibberellins or phytoalexins [2].
CAS REGISTRY NUMBER
COMMENTARY hide
160047-79-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ent-copalyl diphosphate
ent-pimara-8(14),15-diene + diphosphate
show the reaction diagram
ent-copalyl diphosphate
ent-sandaracopimara-8(14),15-diene + diphosphate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ent-copalyl diphosphate
ent-pimara-8(14),15-diene + diphosphate
show the reaction diagram
Q2QQJ5
the enzyme is involved in the biosynthesis of oryzalexins A-F, phytoalexin biosynthesis
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?
additional information
?
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OsKSL10, is induced by either fungal elicitor or UV-irradiation
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0006
ent-copalyl diphosphate
22°C, pH not specified in the publication
additional information
additional information
KM value for substrate syn-copalyl diphosphate is 0.005 mM
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00033
ent-copalyl diphosphate
Oryza sativa
Q2QQJ5
22°C, pH not specified in the publication
additional information
additional information
Oryza sativa
Q2QQJ5
Kcat value for substrate syn-copalyl diphosphate is 0.00083 per s
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.55
ent-copalyl diphosphate
Oryza sativa
Q2QQJ5
22°C, pH not specified in the publication
3716
additional information
additional information
Oryza sativa
Q2QQJ5
Kcat/KM value for substrate syn-copalyl diphosphate is 0.17 per s and mM
2
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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the transcript of OsKS10 increases strongly after UV irradiation
Manually annotated by BRENDA team
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression as glutathione-S-transferase fusion protein