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4.2.3.29: ent-sandaracopimaradiene synthase

This is an abbreviated version!
For detailed information about ent-sandaracopimaradiene synthase, go to the full flat file.

Word Map on EC 4.2.3.29

Reaction

ent-copalyl diphosphate
=
ent-sandaracopimara-8(14),15-diene
+
diphosphate

Synonyms

ent-(sandaraco)pimar-8(14),15-diene synthase, ent-kaurene synthase like 10, ent-sandaracopimaradiene synthase, KS like 10, KS(-like) enzyme, KS(L), KSL10, KSL10i, KSL10j, OrKSL10i, OrKSL10j, OsKS10, OsKSL10, RcKS(L), RcKSL3

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.29 ent-sandaracopimaradiene synthase

Cloned

Cloned on EC 4.2.3.29 - ent-sandaracopimaradiene synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression as glutathione-S-transferase fusion protein
gene KSL10, sequence comparisons and phylogenetic analysis and tree
gene KSL10i, sequence comparisons and phylogenetic analysis and tree
gene KSL10j, sequence comparisons and phylogenetic analysis and tree
molecular phylogenetic analysis and tree, sequence comparisons, overview. The predicted RcKSL3 encodes an active enzyme, isozyme determination and phylogenetic analysis and tree, sequence comparisons, the synthetic RcKS(L) isozyme is truncated to remove the N-terminal plastid-directing transit peptide sequences, it is individually subcloned into compatible expression vectors and coexpressed with either the geranylgeranyl phosphate synthase (GGPS), or the GGPS along with a CPS (EC 5.5.1.13) for recombinant expression in Escherichia coli
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