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4.2.2.25: gellan lyase

This is an abbreviated version!
For detailed information about gellan lyase, go to the full flat file.

Word Map on EC 4.2.2.25

Reaction

Eliminative cleavage of beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyluronate bonds of gellan backbone releasing tetrasaccharides containing a 4-deoxy-4,5-unsaturated D-glucopyranosyluronic acid at the non-reducing end. The tetrasaccharide produced from deacetylated gellan is beta-D-4-deoxy-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp. =

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.25 gellan lyase

Temperature Stability

Temperature Stability on EC 4.2.2.25 - gellan lyase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
pH 7.5, 10 min, 50% loss of activity
55
pH 7.0, 10 min, more than 50% loss of activity
70
half-life: 50 min in absence of substrate. Stable for 2.5 h in presence of substrate
75
at pH 7.2 and a protein concentration above 1 mg/ml large exothermic aggregation occurrs near Tm (75°C) rendering that the unfolding transition is irreversible