Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour-diffusion method at 18°C | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
216000 | - |
capillary gel electrophoresis | Geobacillus stearothermophilus |
220000 | - |
x * 220000, SDS-PAGE | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
gellan | Geobacillus stearothermophilus | gellan lyase cleaves the gellan molecule to tetrasaccharide-repeating units of glucuronic acid-glucose-rhamnose-glucose through the reaction of beta-elimination. Gellan is depolymerized first by gellan lyase and the received tetrasaccharide further hydrolyzed by certain exoenzymes which are found outside the cell in the stationary phase | ? | - |
? | |
gellan | Geobacillus stearothermophilus 98 | gellan lyase cleaves the gellan molecule to tetrasaccharide-repeating units of glucuronic acid-glucose-rhamnose-glucose through the reaction of beta-elimination. Gellan is depolymerized first by gellan lyase and the received tetrasaccharide further hydrolyzed by certain exoenzymes which are found outside the cell in the stationary phase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | P85513 | - |
- |
Geobacillus stearothermophilus 98 | P85513 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gellan | gellan lyase cleaves the gellan molecule to tetrasaccharide-repeating units of glucuronic acid-glucose-rhamnose-glucose through the reaction of beta-elimination. Gellan is depolymerized first by gellan lyase and the received tetrasaccharide further hydrolyzed by certain exoenzymes which are found outside the cell in the stationary phase | Geobacillus stearothermophilus | ? | - |
? | |
gellan | gellan lyase cleaves the gellan molecule to tetrasaccharide-repeating units of glucuronic acid-glucose-rhamnose-glucose through the reaction of beta-elimination | Geobacillus stearothermophilus | ? | - |
? | |
gellan | gellan lyase cleaves the gellan molecule to tetrasaccharide-repeating units of glucuronic acid-glucose-rhamnose-glucose through the reaction of beta-elimination. Gellan is depolymerized first by gellan lyase and the received tetrasaccharide further hydrolyzed by certain exoenzymes which are found outside the cell in the stationary phase | Geobacillus stearothermophilus 98 | ? | - |
? | |
gellan | gellan lyase cleaves the gellan molecule to tetrasaccharide-repeating units of glucuronic acid-glucose-rhamnose-glucose through the reaction of beta-elimination | Geobacillus stearothermophilus 98 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 220000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
at pH 7.2 and a protein concentration above 1 mg/ml large exothermic aggregation occurrs near Tm (75°C) rendering that the unfolding transition is irreversible | Geobacillus stearothermophilus |