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Literature summary for 4.2.2.25 extracted from
- Polysaccharide lyase: molecular cloning of gellan lyase gene and formation of the lyase from a huge precursor protein in Bacillus sp. GL1 (1998), Arch. Biochem. Biophys., 354, 31-39.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus sp. (in: Bacteria) |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | O82833 | - |
- |
| Bacillus sp. (in: Bacteria) GL1 | O82833 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | Bacillus sp. GL1, gellan lyase is first produced as a huge precursor protein (263 kDa) and then the protein is posttranslationally processed into extracellular mature form (140 kDa) through excising a C-terminal peptide of about 120 kDa | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gellan | the recombinant enzyme acts on gellan, especially deacetylated, but is inert on gellan-related polysaccharides such as S-88, welan, rhamsan, and S-198. Xanthan and alginate are not utilized as substrates | Bacillus sp. (in: Bacteria) | ? | - |
? |  |
| gellan | the recombinant enzyme acts on gellan, especially deacetylated, but is inert on gellan-related polysaccharides such as S-88, welan, rhamsan, and S-198. Xanthan and alginate are not utilized as substrates | Bacillus sp. (in: Bacteria) GL1 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
stable below | Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Bacillus sp. (in: Bacteria) |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Bacillus sp. (in: Bacteria) | the enzyme is consitutively produced. Gellan lyase is efficiently induced in presence of xanthan | up |
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