4.2.2.25: gellan lyase
This is an abbreviated version!
For detailed information about gellan lyase, go to the full flat file.
Word Map on EC 4.2.2.25
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4.2.2.25
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polysaccharide
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tetrasaccharide
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depolymerization
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heteropolysaccharide
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beta-d-glucosidase
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glucuronyl
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monosaccharides
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oligosaccharide
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unsaturated
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lyases
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huge
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geobacillus
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bulgarian
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exopolysaccharide
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extracellularly
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rhamnose
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spring
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deacetylated
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stearothermophilus
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nonreducing
- 4.2.2.25
- polysaccharide
- tetrasaccharide
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depolymerization
- heteropolysaccharide
- beta-d-glucosidase
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glucuronyl
- monosaccharides
- oligosaccharide
- unsaturated
- lyases
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huge
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geobacillus
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bulgarian
- exopolysaccharide
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extracellularly
- rhamnose
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spring
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deacetylated
- stearothermophilus
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nonreducing
Reaction
Eliminative cleavage of beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyluronate bonds of gellan backbone releasing tetrasaccharides containing a 4-deoxy-4,5-unsaturated D-glucopyranosyluronic acid at the non-reducing end. The tetrasaccharide produced from deacetylated gellan is beta-D-4-deoxy-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp. =
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Substrates Products
Substrates Products on EC 4.2.2.25 - gellan lyase
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REACTION DIAGRAM
[beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-]n
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + [beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-]n-1
gellan
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highly specific to gellan, especially deacetylated gellan, other gellanrelated polysaccharides such as S-88, welan, rhamsan, and S-198 are inert as substrates
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gellan
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the recombinant enzyme acts on gellan, especially deacetylated, but is inert on gellan-related polysaccharides such as S-88, welan, rhamsan, and S-198. Xanthan and alginate are not utilized as substrates
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-
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gellan
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the recombinant enzyme acts on gellan, especially deacetylated, but is inert on gellan-related polysaccharides such as S-88, welan, rhamsan, and S-198. Xanthan and alginate are not utilized as substrates
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gellan
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highly specific to gellan, especially deacetylated gellan, other gellanrelated polysaccharides such as S-88, welan, rhamsan, and S-198 are inert as substrates
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-
?
gellan
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gellan lyase cleaves the gellan molecule to tetrasaccharide-repeating units of glucuronic acid-glucose-rhamnose-glucose through the reaction of beta-elimination. Gellan is depolymerized first by gellan lyase and the received tetrasaccharide further hydrolyzed by certain exoenzymes which are found outside the cell in the stationary phase
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gellan
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gellan lyase cleaves the gellan molecule to tetrasaccharide-repeating units of glucuronic acid-glucose-rhamnose-glucose through the reaction of beta-elimination
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gellan
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gellan lyase cleaves the gellan molecule to tetrasaccharide-repeating units of glucuronic acid-glucose-rhamnose-glucose through the reaction of beta-elimination. Gellan is depolymerized first by gellan lyase and the received tetrasaccharide further hydrolyzed by certain exoenzymes which are found outside the cell in the stationary phase
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gellan
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gellan lyase cleaves the gellan molecule to tetrasaccharide-repeating units of glucuronic acid-glucose-rhamnose-glucose through the reaction of beta-elimination
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?
gellan
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the enzyme is highly specific to gellan, especially deacetylated gellan, and other gellan-related polysaccharides, such as S-88, welan, rhamsan, and S-198. The viscosity of the gellan solution in the presence of the enzyme decreases and finally reaches a level comparable to that of water
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?
[beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-]n
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + [beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-]n-1
i.e. deacetylated gellan. The purified gellan lyase depolymerizes deacetylated gellan and gives a single oligosaccharide product with a molecular weight of 646 Da, which is determined by fast atom bombardment mass spectrometry. The structure of the product is determined by the combination of mass spectrometry, HPLC analysis, and high-resolution proton nuclear magnetic resonance spectroscopy to be a tetrasaccharide of glucuronyl-glucosyl-rhamnosyl-glucose with unsaturated glucuronic acid at the nonreducing terminal
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?
[beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-]n
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + [beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-]n-1
i.e. deacetylated gellan. The purified gellan lyase depolymerizes deacetylated gellan and gives a single oligosaccharide product with a molecular weight of 646 Da, which is determined by fast atom bombardment mass spectrometry. The structure of the product is determined by the combination of mass spectrometry, HPLC analysis, and high-resolution proton nuclear magnetic resonance spectroscopy to be a tetrasaccharide of glucuronyl-glucosyl-rhamnosyl-glucose with unsaturated glucuronic acid at the nonreducing terminal
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