4.2.2.18: inulin fructotransferase (DFA-III-forming)
This is an abbreviated version!
For detailed information about inulin fructotransferase (DFA-III-forming), go to the full flat file.
Word Map on EC 4.2.2.18
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4.2.2.18
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arthrobacter
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2.4.1.93
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difructose
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globiformis
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synthesis
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sweet
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smallest
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fructooligosaccharides
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low-calorie
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oligosaccharide
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1-kestose
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disaccharide
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chitopearl
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aurescens
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deae-toyopearl
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inulobiose
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broth
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sweetener
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biotechnology
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raffinose
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super
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levans
- 4.2.2.18
- arthrobacter
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2.4.1.93
- difructose
- globiformis
- synthesis
-
sweet
-
smallest
- fructooligosaccharides
-
low-calorie
- oligosaccharide
- 1-kestose
- disaccharide
-
chitopearl
- aurescens
-
deae-toyopearl
- inulobiose
- broth
-
sweetener
- biotechnology
- raffinose
-
super
- levans
Reaction
Synonyms
Achl_2902, BsIFTase, di-D-fructofuranose 1,2': 2,3' dianhydride [DFA III]-producing IFTase, EC 2.4.1.93, fructotransferase, inulin (depolymerizing), IFTase, IFTase (DFA III-producing), IFTaseIII, inulase II, inulin D-fructosyl-D-fructosyltransferase (1,2':2,3'-dianhydride-forming), inulin fructotransferase (depolymerizing), inulin fructotransferase (depolymerizing, difructofuranose-1,2':2,3'-dianhydride-forming), inulin fructotransferase (DFA III-producing), inulin fructotransferase (DFA-III-producing), inulin fructotransferase (DFAIII-producing), inulinase II
ECTree
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Application
Application on EC 4.2.2.18 - inulin fructotransferase (DFA-III-forming)
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biotechnology
synthesis
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improved enzyme for large scale production of low-calorie sweet food additive
biotechnology
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large scale production of sweet food additive DFA III via recombinant expression in E. coli, genetic engineering of the enzyme for enlarged thermotolerance, immobilization of the enzyme on alginate beads
biotechnology
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improved enzyme for large scale production of low-calorie sweet food additive
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enzyme immobilized on Chitopearl BCW 3510 can be used 10 times without a significant loss in activity
synthesis
production of dry powder of inulin fructotransferase from fermented liquor. Lyophilized enzyme shows good storage stability
synthesis
use of enzyme in an ultrafiltration membrane bioreactor for the production of difructose anhydrides III from enzymatic conversion of inulin. The enzyme can be continuously used for six runs in the ultrafiltration membrane bioreactor. When the substrate concentration is 100 g/l, the concentration of difructose anhydrides III is about 78.4 g/l, while the productivity and purity of difructose anhydrides III can attain about 2385 and 92%, respectively
synthesis
WP_018778058
conversion ratio of inulin to DFA III reaches 81% when 100 g/l inulin is catalyzed by 80 nM recombinant enzyme for 20 min at pH 6.5 and 55°C
synthesis
use of high hydrostatic pressure to improve the catalytic efficiency. The maximum activity of IFTase is obtained under 200 MPa at 60°C. High hydrostatic pressure lowers the energy barrier of the enzymatic reaction and decreases the volume between the reactants and the transition state. Under this condition, the optimal pH for the enzymatic reaction shifts from 5.5 to 6.0. The activity is further enhanced by 65.2% in the presence of 1.5 M NaCl
synthesis
using expression of IFTase in Pichia pastoris under the control of the formaldehyde dehydrogenase 1 promoter, efficient secretion with four substrate fed-batch strategies in a 3-L fermenter can be achieved. The cofeeding induction strategy with methylamine hydrochloride and methanol achieves the maximum extracellular IFTase activity of 62.72 U/ml. Carbon sources such as glucose and glycerol can also be utilised using methylamine hydrochloride induction but should be strictly controlled at low concentrations of 0.5-1.5 % (v/v) and 1-1.5 % (w/v), respectively
synthesis
-
production of dry powder of inulin fructotransferase from fermented liquor. Lyophilized enzyme shows good storage stability
-
synthesis
-
use of enzyme in an ultrafiltration membrane bioreactor for the production of difructose anhydrides III from enzymatic conversion of inulin. The enzyme can be continuously used for six runs in the ultrafiltration membrane bioreactor. When the substrate concentration is 100 g/l, the concentration of difructose anhydrides III is about 78.4 g/l, while the productivity and purity of difructose anhydrides III can attain about 2385 and 92%, respectively
-
synthesis
-
using expression of IFTase in Pichia pastoris under the control of the formaldehyde dehydrogenase 1 promoter, efficient secretion with four substrate fed-batch strategies in a 3-L fermenter can be achieved. The cofeeding induction strategy with methylamine hydrochloride and methanol achieves the maximum extracellular IFTase activity of 62.72 U/ml. Carbon sources such as glucose and glycerol can also be utilised using methylamine hydrochloride induction but should be strictly controlled at low concentrations of 0.5-1.5 % (v/v) and 1-1.5 % (w/v), respectively
-
synthesis
-
enzyme immobilized on Chitopearl BCW 3510 can be used 10 times without a significant loss in activity
-