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4.2.2.18: inulin fructotransferase (DFA-III-forming)

This is an abbreviated version!
For detailed information about inulin fructotransferase (DFA-III-forming), go to the full flat file.

Word Map on EC 4.2.2.18

Reaction

[inulin]n
=
[inulin]n-2
+
alpha-D-fructofuranose beta-D-fructofuranose 1,2':2,3'-dianhydride

Synonyms

Achl_2902, BsIFTase, di-D-fructofuranose 1,2': 2,3' dianhydride [DFA III]-producing IFTase, EC 2.4.1.93, fructotransferase, inulin (depolymerizing), IFTase, IFTase (DFA III-producing), IFTaseIII, inulase II, inulin D-fructosyl-D-fructosyltransferase (1,2':2,3'-dianhydride-forming), inulin fructotransferase (depolymerizing), inulin fructotransferase (depolymerizing, difructofuranose-1,2':2,3'-dianhydride-forming), inulin fructotransferase (DFA III-producing), inulin fructotransferase (DFA-III-producing), inulin fructotransferase (DFAIII-producing), inulinase II

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.18 inulin fructotransferase (DFA-III-forming)

Crystallization

Crystallization on EC 4.2.2.18 - inulin fructotransferase (DFA-III-forming)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystals are obtained at 20°C by hanging drop vapour-diffusion method using 0.1 M Na HEPES pH 7.5 buffer containing 1.5 M lithium sulfate as a precipitant. Crystals of the recombinant wild-type enzyme diffract to better than 1.5 A at -173°C. The crystals belong to space group R32, with unit-cell parameters a = b = 90.02 A, c = 229.82 A in the hexagonal axes. Selenomethionine-derivative crystals belong to a different space group, C2, with unit-cell parameters a = 159.32, cb = 91.92, c = 92.58 A, beta = 125.06. The C2 selenomethionine-derived crystal contains three molecules per asymmetric unit
-
homology modeling of structure. Residues Ser309 and Ser333 may play key roles in the structural stability
WP_018778058
hanging drop vapor diffusion method
-