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Literature summary for 4.2.2.18 extracted from

  • Ueda, M.; Sashida, R.; Morimoto, Y.; Ohkishi, H.
    Purification of inulin fructotransferase (DFA I-producing) from Arthrobacter sp. MCI2493 and production of DFA I from inulin by the enzyme (1994), Biosci. Biotechnol. Biochem., 58, 574-575.
    View publication on PubMed
Search for more literature for 4.2.2.18

Application

Application Comment Organism
biotechnology improved enzyme for large scale production of low-calorie sweet food additive Arthrobacter sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Arthrobacter sp.
-
-
extracellular
-
 Streptomyces sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 2 * 36000, SDS-PAGE Streptomyces sp.
40000
-
 gel filtration Arthrobacter sp.
46000
-
 gel filtration Arthrobacter globiformis
70000
-
 gel filtration Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis
-
-
-
Arthrobacter globiformis S14-3
-
-
-
Arthrobacter sp.
-
-
-
Arthrobacter sp. MCI-2493
-
-
-
Streptomyces sp.
-
-
-
Streptomyces sp. MCI-2524
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arthrobacter globiformis
-
 Streptomyces sp.
from strain MCI-2493 Arthrobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Arthrobacter sp.
-
culture medium
-
 Streptomyces sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
81.5
-
 purified enzyme Arthrobacter globiformis
256
-
 purified enzyme Streptomyces sp.
384
-
 purified enzyme Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin cf. EC 2.4.1.200 Arthrobacter globiformis di-D-fructofuranose 1,2':2,1' dianhydride + ? i.e. DFA I ?
inulin cf. EC 2.4.1.200 Arthrobacter sp. di-D-fructofuranose 1,2':2,1' dianhydride + ? i.e. DFA I ?
inulin cf. EC 2.4.1.200 Streptomyces sp. di-D-fructofuranose 1,2':2,1' dianhydride + ? i.e. DFA I ?
inulin cf. EC 2.4.1.200 Arthrobacter sp. di-D-fructofuranose 1,2':2,1' dianhydride + ? other products are: nystose and 1-F-fructofuranosyl-nystose ?
inulin cf. EC 2.4.1.200 Arthrobacter sp. MCI-2493 di-D-fructofuranose 1,2':2,1' dianhydride + ? i.e. DFA I ?
inulin cf. EC 2.4.1.200 Arthrobacter sp. MCI-2493 di-D-fructofuranose 1,2':2,1' dianhydride + ? other products are: nystose and 1-F-fructofuranosyl-nystose ?
inulin cf. EC 2.4.1.200 Streptomyces sp. MCI-2524 di-D-fructofuranose 1,2':2,1' dianhydride + ? i.e. DFA I ?
inulin cf. EC 2.4.1.200 Arthrobacter globiformis S14-3 di-D-fructofuranose 1,2':2,1' dianhydride + ? i.e. DFA I ?

Subunits

Subunits Comment Organism
dimer
-
 Arthrobacter sp.
dimer 2 * 36000, SDS-PAGE Streptomyces sp.
monomer 1 * 40000, SDS-PAGE Arthrobacter sp.
monomer 1 * 46000, SDS-PAGE Arthrobacter globiformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Arthrobacter globiformis
50
-
-
 Arthrobacter sp.
65
-
-
 Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 more than 80% activity remains after 30 min, pH 6.0 Arthrobacter sp.
80
-
-
 Arthrobacter globiformis
80
-
 most of the activity is lost after 30 min Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Arthrobacter globiformis
6
-
-
 Arthrobacter sp.
6
-
-
 Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
-
 Arthrobacter globiformis
4
-
 30°C, 30 min, stable Arthrobacter sp.