4.2.2.16: levan fructotransferase (DFA-IV-forming)
This is an abbreviated version!
For detailed information about levan fructotransferase (DFA-IV-forming), go to the full flat file.
Word Map on EC 4.2.2.16
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4.2.2.16
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arthrobacter
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difructose
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ureafaciens
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levanbiose
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inulin
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nicotinovorans
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disaccharide
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microbacterium
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oligosaccharides
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fructans
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synthesis
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gel-filtration
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tetraose
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non-reducing
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transfructosylation
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oligofructans
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sweetener
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iptg
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monosaccharide
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fructooligosaccharides
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levanase
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biotechnology
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medicine
- 4.2.2.16
- arthrobacter
- difructose
- ureafaciens
- levanbiose
- inulin
- nicotinovorans
- disaccharide
- microbacterium
- oligosaccharides
- fructans
- synthesis
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gel-filtration
-
tetraose
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non-reducing
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transfructosylation
- oligofructans
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sweetener
- iptg
- monosaccharide
- fructooligosaccharides
- levanase
- biotechnology
- medicine
Reaction
Synonyms
2,6-beta-D-fructan D-fructosyl-D-fructosyltransferase (forming di-beta-D-fructofuranose 2,6':2',6-dianhydride), fructotransferase, levan, FTF, levan fructotransferase, levan fructotransferase (DFA-IV-forming), LFT, LFTase, LftM
ECTree
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Application
Application on EC 4.2.2.16 - levan fructotransferase (DFA-IV-forming)
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biotechnology
medicine
synthesis
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production of Arthrobacter levan fructotransferase from recombinant Escherichia coli at high levels via secretion directed by a novel N-terminal motif, TMITNSSSVP. A large amount of extracellular recombinant levan fructotransferase can be produced with high productivity through cost-effective processes
biotechnology
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production of Arthrobacter levan fructotransferase from recombinant Escherichia coli at high levels via secretion directed by a novel N-terminal motif, TMITNSSSVP. A large amount of extracellular recombinant levan fructotransferase can be produced with high productivity through cost-effective processes
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the product of the enzymatic reaction di-D-fructose-2,6':2',6 dianhydride stimulates calcium absorption mainly in small intestine of rats and has a practical physiological property in preventing osteoporosis
medicine
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the product of the enzymatic reaction di-D-fructose-2,6':2',6 dianhydride stimulates calcium absorption mainly in small intestine of rats and has a practical physiological property in preventing osteoporosis
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synthesis
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development of a di-D-fructofranosyl-2,6':2',6-anhydride production system with single culture of Bacillus subtilis directly from sucrose. This system can avoid the purification procedure of levan in which organic solvent is used for precipitation. The levan fructotransferase gene is cloned from Arthrobacter nicotinovorans GS-9 and expressed in levan producing Bacillus subtilis 168. LFTase activity is detected in the culture supernatant of the transformant with maximal activity of 0.062 U/ml after 15.5 h post induction. Then sucrose is added as substrate and incubated. About 78 h after addition of sucrose, 20.5 g/l of di-D-fructofranosyl-2,6':2',6-anhydride is produced from 139.3 g/l of sucrose consumed. The yield of di-D-fructofranosyl-2,6':2',6-anhydride from sucrose is 14.7 wt.%
synthesis
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enzymatic production of ascorbic acid 2-fructoside, that can be applied to cosmetics, food products, and pharmaceuticals
synthesis
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development of a di-D-fructofranosyl-2,6':2',6-anhydride production system with single culture of Bacillus subtilis directly from sucrose. This system can avoid the purification procedure of levan in which organic solvent is used for precipitation. The levan fructotransferase gene is cloned from Arthrobacter nicotinovorans GS-9 and expressed in levan producing Bacillus subtilis 168. LFTase activity is detected in the culture supernatant of the transformant with maximal activity of 0.062 U/ml after 15.5 h post induction. Then sucrose is added as substrate and incubated. About 78 h after addition of sucrose, 20.5 g/l of di-D-fructofranosyl-2,6':2',6-anhydride is produced from 139.3 g/l of sucrose consumed. The yield of di-D-fructofranosyl-2,6':2',6-anhydride from sucrose is 14.7 wt.%
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