4.2.2.16: levan fructotransferase (DFA-IV-forming)
This is an abbreviated version!
For detailed information about levan fructotransferase (DFA-IV-forming), go to the full flat file.
Word Map on EC 4.2.2.16
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4.2.2.16
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arthrobacter
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difructose
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ureafaciens
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levanbiose
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inulin
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nicotinovorans
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disaccharide
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microbacterium
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oligosaccharides
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fructans
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synthesis
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gel-filtration
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tetraose
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non-reducing
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transfructosylation
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oligofructans
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sweetener
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iptg
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monosaccharide
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fructooligosaccharides
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levanase
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biotechnology
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medicine
- 4.2.2.16
- arthrobacter
- difructose
- ureafaciens
- levanbiose
- inulin
- nicotinovorans
- disaccharide
- microbacterium
- oligosaccharides
- fructans
- synthesis
-
gel-filtration
-
tetraose
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non-reducing
-
transfructosylation
- oligofructans
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sweetener
- iptg
- monosaccharide
- fructooligosaccharides
- levanase
- biotechnology
- medicine
Reaction
Synonyms
2,6-beta-D-fructan D-fructosyl-D-fructosyltransferase (forming di-beta-D-fructofuranose 2,6':2',6-dianhydride), fructotransferase, levan, FTF, levan fructotransferase, levan fructotransferase (DFA-IV-forming), LFT, LFTase, LftM
ECTree
4.2.2.16 levan fructotransferase (DFA-IV-forming)Advanced search results
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results (Substrates Products
Substrates Products on EC 4.2.2.16 - levan fructotransferase (DFA-IV-forming)
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ascorbic acid + levan
ascorbic acid 2-fructoside + ?
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transfructosylation. When 35% of ascorbic acid and 2% of levan are incubated with LFTase of 0.5 unit/g levan at 37°C for 85 h, a maximum 52 g/l of ascorbic acid 2-fructoside is produced
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-
?
phlein
di-D-fructose-2,6':2',6 dianhydride
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while it degrades Phleum pratense phlein more than 90%, formation of 6% reducing sugars as D-fructose
and two or three minor fructooligosaccharides
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
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levan from Zymomonas mobilis: conversion yield 28.4% after 24 h. Levan from Serratia: conversion yield: 28.4% after 24 h
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?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
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levan from Zymomonas mobilis: conversion yield 28.4% after 24 h. Levan from Serratia: conversion yield: 28.4% after 24 h
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?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
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-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
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-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
-
-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
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-
di-D-fructose-2,6':2',6 dianhydride is the main product, 75%. Fructose, levanbiose, and two unidentified oligosaccharides are minor products
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
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the enzyme is specifically induced by levan
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?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
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-
di-D-fructose-2,6':2',6 dianhydride is the main product, 75%. Fructose, levanbiose, and two unidentified oligosaccharides are minor products
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
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the enzyme is specifically induced by levan
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?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
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-
-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
-
-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
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the enzyme degrades Bacillus mesentericus levan up to 40% leaving the levan fragment at the limit of the enzymatic degradation, formation of 5% reducing sugars as D-fructose
and two or three minor fructooligosaccharides
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
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levan from Zymomonas mobilis, conversion yield is 35%
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?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
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levan from Zymomonas mobilis, conversion yield is 35%
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?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
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-
-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
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-
-
-
?
levan
di-D-fructofuranose-2,6':2',6 dianhydride + ?
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and small amounts of several oligosaccharides and free fructose
?
levan
di-D-fructofuranose-2,6':2',6 dianhydride + ?
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and small amounts of several oligosaccharides and free fructose
?
levan
di-D-fructose-2,6':2',6 dianhydride + fructose + limited levan
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-
di-D-fructose-2,6':2',6 dianhydride (49.1%) + fructose (3.5%) + limited levan (16.2%)
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?
levan
di-D-fructose-2,6':2',6 dianhydride + fructose + limited levan
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-
di-D-fructose-2,6':2',6 dianhydride (49.1%) + fructose (3.5%) + limited levan (16.2%)
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?
raffinose + D-galactopyranose
?
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transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside
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r
raffinose + D-galactopyranose
?
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transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside
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r
stachyose + D-galactopyranose
?
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transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside
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r
stachyose + D-galactopyranose
?
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transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside
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r
sucrose + D-galactopyranose
?
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transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside
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r
sucrose + D-galactopyranose
?
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transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside
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r
additional information
?
-
-
no activity with dextran, soluble starch, inulin, maltose, sucrose, raffinose, 1-kestose, nystose and 1F-fructofuranosyl nystose
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?
additional information
?
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the enzyme never hydrolyzes beta-2,1-fructosyl-linked fructans, such as inulin, as well as oligosaccharides such as 1-kestose and nystose
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?
additional information
?
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no activity with dextran, soluble starch, inulin, maltose, sucrose, raffinose, 1-kestose, nystose and 1F-fructofuranosyl nystose
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?
additional information
?
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the enzyme never hydrolyzes beta-2,1-fructosyl-linked fructans, such as inulin, as well as oligosaccharides such as 1-kestose and nystose
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?
additional information
?
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-
no formation of fructose, levanbiose, nor difructose anhydride IV from bifurcose and [O-beta-D-Fru(f)-(2-1)]-O-[beta-D-Fru(f)-(2-6)]2-O-beta-D-Fru(f)-(2-1)-alpha-D-G(p)
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?
additional information
?
-
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no activity with inulin, levanbiose, sucrose, dextran or starch
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?
additional information
?
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no activity with inulin, levanbiose, sucrose, dextran or starch
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?
