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4.2.2.16: levan fructotransferase (DFA-IV-forming)

This is an abbreviated version!
For detailed information about levan fructotransferase (DFA-IV-forming), go to the full flat file.

Word Map on EC 4.2.2.16

Reaction

[levan]n
=
di-beta-D-fructofuranose 2,6':2',6-dianhydride
 +
[Levan]n-2

Synonyms

2,6-beta-D-fructan D-fructosyl-D-fructosyltransferase (forming di-beta-D-fructofuranose 2,6':2',6-dianhydride), fructotransferase, levan, FTF, levan fructotransferase, levan fructotransferase (DFA-IV-forming), LFT, LFTase, LftM

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.16 levan fructotransferase (DFA-IV-forming)

Cloned

Cloned on EC 4.2.2.16 - levan fructotransferase (DFA-IV-forming)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Bacillus subtilis strain 168
-
expressed in Escherichia coli as a His-tagged fusion protein
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed with N-terminal fusion of a LacZ-derived secretion motif TMITNSSSVP using lac promoter system in recombinant Escherichia coli JM109[pUDF-A8]
-
expression in Escherichia coli
expression in Escherichia coli. An Escherichia coli transformant carrying the plasmid pLFT-BB1 expresses six times as much activity of levan fructotransferase as that of the original strain Arthrobacter nicotinovorans GS-9
-
levan fructotransferase gene is cloned from Arthrobacter nicotinovorans GS-9 and expressed in levan producing Bacillus subtilis 168
-
overexpression in Escherichia coli
-
subcloned into a high-expression vector, pET-29b, and the recombinant enzyme with a tag of six histidine is overexpressed in Escherichia coli